Email this article THE EXPRESSION AND POLYMORPHISM INFLUENCE OF GENES, ENCODING THE GABA-RECEPTORS, ON SEVERITY OF THE ETHANOL DEPRESSING ACTION IN RATS
- Authors: Osechkina N.S.1, Nazarov G.V.2, Bonitenko E.Y.1, Ivanov M.B.1, Kashuro V.A.1, Lapina N.V.1, Babkin A.V.2, Berdinskih I.S.2, Melekhova A.S.1, Voytsekhovich K.O.1, Lisitskiy D.S.1, Makarova N.V.1, Kashina T.V.1
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Affiliations:
- Federal State Budgetary Science Institution «Institute of Toxicology» of Federal Medico-Biological Agency
- Scientific Research Test Institute of Military Medicine, S.M. Kirov Medical Military Academy
- Issue: No 6 (2014)
- Pages: 22-27
- Section: Articles
- Published: 22.12.2014
- URL: https://rjsocmed.com/0869-7922/article/view/641383
- DOI: https://doi.org/10.15690/vramn545
- ID: 641383
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Abstract
Expression and polymorphism of encoding the Gaba-receptors of genes, which determine the severity of the depressing effect of ethanol in white outbred rats were studied. In the course of the experiment, genotyping of animals was made using 16 single-nucleotide replacements in genes encoding the GABA. The genetic diversity of allelic variants was revealed, based on 11 polymorphisms. It was found out that in rats with the genotype homozygous variant TT (rs 197587817) the expression level of gene Gabra l (in brain 8 h after administration of ethanol) was authentically lower (2-fold) as compared to the heterozygous genotype CT. An authentic association of the gene Gabrb1 expression level (p<0.05) with the severity degree of the ethanol depressing effect on the nervous system in rats 8 h after administration of the toxicant was demonstrated.
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About the authors
Natalya Sergeevna Osechkina
Federal State Budgetary Science Institution «Institute of Toxicology» of Federal Medico-Biological Agency
Author for correspondence.
Email: noemail@neicon.ru
Младший научный сотрудник лаборатории биохимической токсикологии и фармакологии ФГБУН «Институт токсикологии» Федерального медико-биологического агентства России, 192019, г. Санкт-Петербург
e-mail: dunats@rambler.ru
Russian FederationGeorgiy Valerevich Nazarov
Scientific Research Test Institute of Military Medicine, S.M. Kirov Medical Military Academy
Email: noemail@neicon.ru
Доктор химических наук, доцент, начальник отдела научно-исследовательского испытательного института военной медицины Военно-медицинской академии им. С.М. Кирова, 195043, г. Санкт-Петербург
e-mail: denis-100@list.ru
Russian FederationEvgeniy Yur’evich Bonitenko
Federal State Budgetary Science Institution «Institute of Toxicology» of Federal Medico-Biological Agency
Email: noemail@neicon.ru
Доктор медицинских наук, директор ФГБУН «Институт токсикологии» Федерального медико-биологического агентства России, 192019, г. Санкт-Петербург
e-mail: eu_bonitenko@mail.ru
Russian FederationMaksim Borisovich Ivanov
Federal State Budgetary Science Institution «Institute of Toxicology» of Federal Medico-Biological Agency
Email: noemail@neicon.ru
Доктор медицинских наук, заместитель директора по научной работе ФГБУН «Институт токсикологии» Федерального медико-биологического агентства России, 192019, г. Санкт-Петербург,
e-mail: m.b.ivanov@toxicology.ru
Russian FederationVadim Anatol’evich Kashuro
Federal State Budgetary Science Institution «Institute of Toxicology» of Federal Medico-Biological Agency
Email: noemail@neicon.ru
Доктор медицинских наук, заведующий лабораторией биохимической токсикологии и фармакологии ФГБУН «Институт токсикологии» Федерального медико-биологического агентства России, 192019, г. Санкт-Петербург
е-mail: kashuro@yandex.ru
Russian FederationNataliya Vadimovna Lapina
Federal State Budgetary Science Institution «Institute of Toxicology» of Federal Medico-Biological Agency
Email: noemail@neicon.ru
Кандидат медицинских наук, ведущий научный сотрудник лаборатории токсикологии ФГБУН «Институт токсикологии» Федерального медико-биологического агентства России, 192019, г. Санкт-Петербург,
е-mail: lapina2005@inbox.ru
Russian FederationAleksandr Vladimirovich Babkin
Scientific Research Test Institute of Military Medicine, S.M. Kirov Medical Military Academy
Email: noemail@neicon.ru
Научный сотрудник научно-исследовательского испытательного института военной медицины Военно-медицинской академии им. С.М. Кирова, 195043, г. Санкт-Петербург
е-mail: babkinnik@yandex.ru
Russian FederationIrina Sergeevna Berdinskih
Scientific Research Test Institute of Military Medicine, S.M. Kirov Medical Military Academy
Email: noemail@neicon.ru
Научный сотрудник научно-исследовательского испытательного института военной медицины Военно-медицинской академии им. С.М. Кирова, 195043, г. Санкт-Петербург
е-mail: irina_berdinskih@mail.ru
Russian FederationAleksandra Sergeevna Melekhova
Federal State Budgetary Science Institution «Institute of Toxicology» of Federal Medico-Biological Agency
Email: noemail@neicon.ru
Младший научный сотрудник лаборатории психофармакологии ФГБУН «Институт токсикологии» Федерального медико-биологического агентства России, 192019, г. Санкт-Петербург,
е-mail: melehovaalexandra@gmail.com
Russian FederationKarina Olegovna Voytsekhovich
Federal State Budgetary Science Institution «Institute of Toxicology» of Federal Medico-Biological Agency
Email: noemail@neicon.ru
Младший научный сотрудник лаборатории психофармакологии ФГБУН «Институт токсикологии» Федерального медико-биологического агентства России, 192019, г. Санкт-Петербург
е-mail: carvoice@gmail.com
Russian FederationDmitriy Sergeevich Lisitskiy
Federal State Budgetary Science Institution «Institute of Toxicology» of Federal Medico-Biological Agency
Email: noemail@neicon.ru
Кандидат биологических наук, научный сотрудник лаборатории психофармакологии ФГБУН «Институт токсикологии» Федерального медико-биологического агентства России, 192019, г. Санкт-Петербург,
е-mail: lisitskiydmitriy@gmail.com
Russian FederationNatal’ya Vasil’evna Makarova
Federal State Budgetary Science Institution «Institute of Toxicology» of Federal Medico-Biological Agency
Email: noemail@neicon.ru
Доктор медицинских наук, научный сотрудник лаборатории психофармакологии ФГБУН «Институт токсикологии» Федерального медико-биологического агентства России, 192019, г. Санкт-Петербург
е-mail: makarova1953@list.ru
Russian FederationTat’yana Vladimirovna Kashina
Federal State Budgetary Science Institution «Institute of Toxicology» of Federal Medico-Biological Agency
Email: noemail@neicon.ru
Ветеринарный врач лаборатории психофармакологии ФГБУН «Институт токсикологии» Федерального медико-биологического агентства России, 192019, г. Санкт-Петербург
е-mail: tat.t@mail.ru
Russian FederationReferences
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