Email this article Morphofunctional changes in the animal organisms under oral exposure to anatoxin-a in subchronic experimental settings
- Authors: Sinitsyna O.O.1, Turbinskii V.V.1, Pushkareva M.V.1, Kuz N.V.1, Masaltsev G.V.1, Shiryaeva M.A.1, Ryashentseva T.M.1, Valtseva E.A.1, Vostrikova M.V.1
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Affiliations:
- Federal Budgetary Institution of Science “Federal Scientific Center of Hygiene named after F.F. Erisman” of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
- Issue: No 6 (2024)
- Pages: 336-347
- Section: Original articles
- Published: 15.12.2024
- URL: https://rjsocmed.com/0869-7922/article/view/646168
- DOI: https://doi.org/10.47470/0869-7922-2024-6-336-347
- EDN: https://elibrary.ru/jltlsi
- ID: 646168
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Abstract
Introduction. Anatoxin-a (2-acetyl-9-azabicyclo[4.2.1]non-2-ene) produced by blue-green algae of the genera Dolichospermum (Anabaena), Aphanizomenon, Cylindrospermum, Oscillatoria, Planktothrix and Raphidiopsis, which are widely spread in the reservoir waters, including those serving as drinking water supply sources for the population. At the same time, the lack of information on the toxic effect of low doses of anatoxin-a (ATX-a) on the morphofunctional condition of warm-blooded animals’ internal organs under prolonged oral intake into the body remains poorly researched.
The aim of the research was to study morphofunctional changes in internal organs under the influence of anatoxin-a on the white rat bodies at intragastric intake in subchronic pilot experiments.
Material and methods. ATX-a as a certified reference sample in 1% acetic acid solution was dosed daily to male white rats in doses of 0.01; 0.1 and 1.0 µg/kg for 90 days. Morphofunctional changes in 13 internal organs were assessed: thyroid gland, thymus, heart, lung, stomach, liver, spleen, pancreas, ileum, colon, kidney, adrenal glands, testes.
Results. It was established that administration of ATX-a in the animals’ body at a dose of 1.0 µg/kg b.w. was followed by significant changes in the same organs compared to the control: testes (2.7-fold increase in the number of spermatogenic cells in some seminal tubules), thymus (4-fold increase in the proportion of lipomatous areas), stomach (3.5-fold increase in hypersecretion of intrinsic glands, 4-fold – disturbance of borders between mucosa and submucosa, 2.7 times – thinning of connective tissue fibres), colon (hypersecretion and 2.4 times increase of blood vessels), pancreas (2.3 times increase of Langerhans islets fibrosis). When exposed to ATX-a at doses of 0.1 and 1.0 µg/kg b.w. in the adrenal glands, an increase in ectopy by 3.3 and 2.5 times, respectively, compared to the control was observed.
Limitations. The study is limited by the intragastric intake conditions and the use of one species and sex of warm-blooded animals in the experiment.
Conclusion. Morphofunctional changes were revealed in the testes, thymus, stomach, colon, pancreas, adrenal glands of white rats under oral exposure to ATX-a in a subchronic experiment.
Compliance with ethical standards. A positive conclusion was received from the Commission on Biomedical Ethics of the Federal Budgetary Institution of Science “Federal Scientific Center of Hygiene named after F.F. Erisman” (Protocol No. 03/22 dated 11/21/2022).
Authors contribution:
Sinitsyna O.O. – concept and design of research, data processing, writing and editing of text;
Turbinskii V.V. – concept and design of research, text editing;
Pushkareva M.V., Kuz N.V. – collection and data processing, text editing;
Masaltsev G.V., Shiryaeva M.A., Ryashentseva T.M., Vostrikova M.V. – data collection and processing;
Valtseva E.A. – mathematical processing of research results.
All authors are responsible for the integrity of all parts of the article, the approval of the final version of the article.
Funding. The study had no sponsorship.
Conflict of interest. The authors declare no conflict of interest.
Accepted: July 08, 2024 / Revised: August 26, 2024 / Received: November 12, 2024 / Published: December 28, 2024
About the authors
Oxana O. Sinitsyna
Federal Budgetary Institution of Science “Federal Scientific Center of Hygiene named after F.F. Erisman” of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Email: sinitsyna.oo@fncg.ru
ORCID iD: 0000-0002-0241-0690
Doctor of Medical Sciences, Professor, Corresponding Member of the Russian Academy of Sciences, Deputy Director for Scientific Work of the Federal Scientific Center of Hygiene named after F.F. Erisman of the Federal Service for Supervision of Consumer Rights Protection and Human Wellbeing, 141014, Moscow region, Mytishchi, Russian Federation
e-mail: sinitsyna.oo@fncg.ru
Viktor V. Turbinskii
Federal Budgetary Institution of Science “Federal Scientific Center of Hygiene named after F.F. Erisman” of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Email: turbinskii.vv@fncg.ru
ORCID iD: 0000-0001-7668-9324
Doctor of Medical Sciences, Associate Professor, Head of the Department of Water Hygiene of the Federal Scientific Center of Hygiene named after F.F. Erisman of the Federal Service for Supervision of Consumer Rights Protection and Human Wellbeing, 141014, Moscow region, Mytishchi, Russian Federation
e-mail: turbinskii.vv@fncg.ru
Maria V. Pushkareva
Federal Budgetary Institution of Science “Federal Scientific Center of Hygiene named after F.F. Erisman” of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Email: pushkareva.mv@fncg.ru
ORCID iD: 0000-0002-5932-6350
Doctor of Medical Sciences, Professor, Chief Researcher at the Department of Water Hygiene of the Federal Scientific Center of Hygiene named after F.F. Erisman, of the Federal Service for Supervision of Consumer Rights Protection and Human Wellbeing, 141014, Moscow region, Mytishchi, Russian Federation
e-mail: pushkareva.mv@fncg.ru
Nadezhda V. Kuz
Federal Budgetary Institution of Science “Federal Scientific Center of Hygiene named after F.F. Erisman” of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Email: noemail@neicon.ru
ORCID iD: 0000-0002-7573-0185
Researcher at the Department of Water Hygiene of the Federal Scientific Center of Hygiene named after F.F. Erisman of Federal Service for Supervision of Consumer Rights Protection and Human Wellbeing, 141014, Moscow region, Mytishchi, Russian Federation
Gleb V. Masaltsev
Federal Budgetary Institution of Science “Federal Scientific Center of Hygiene named after F.F. Erisman” of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Email: masalcev.gv@fncg.ru
ORCID iD: 0000-0003-1539-1633
Candidate of Biological Sciences, Head of the Toxicology Department, Federal Scientific Center of Hygiene named after F.F. Erisman of Federal Service for Supervision of Consumer Rights Protection and Human Wellbeing, 141014, Moscow region, Mytishchi, Russian Federation
e-mail: masalcev.gv@fncg.ru
Margarita A. Shiryaeva
Federal Budgetary Institution of Science “Federal Scientific Center of Hygiene named after F.F. Erisman” of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Email: shiryaeva.ma@fncg.ru
ORCID iD: 0000-0001-8019-1203
Junior Researcher at the Water Hygiene Department, Federal Scientific Center of Hygiene named after F.F. Erisman of Federal Service for Supervision of Consumer Rights Protection and Human Wellbeing, 141014, Moscow region, Mytishchi, Russian Federation
e-mail: shiryaeva.ma@fncg.ru
Tatyana M. Ryashentseva
Federal Budgetary Institution of Science “Federal Scientific Center of Hygiene named after F.F. Erisman” of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Email: ryashenceva.tm@fncg.ru
ORCID iD: 0000-0003-0899-3505
Junior Researcher at the Water Hygiene Department, Federal Scientific Centre of Hygiene named after F.F. Erisman of Federal Service for Supervision of Consumer Rights Protection and Human Wellbeing, 141014, Moscow region, Mytishchi, Russian Federation
e-mail: ryashenceva.tm@fncg.ru
Elena A. Valtseva
Federal Budgetary Institution of Science “Federal Scientific Center of Hygiene named after F.F. Erisman” of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Email: valtseva.ea@fncg.ru
ORCID iD: 0000-0001-5468-5381
Candidate of Biological Sciences, Chief Specialist of Organizational and Methodological Department of the Federal Scientific Centre of Hygiene named after F.F. Erisman. Of Federal Service for Supervision of Consumer Rights Protection and Human Wellbeing, Moscow region, Mytishchi, Russian Federation
e-mail: valtseva.ea@fncg.ru
Marina V. Vostrikova
Federal Budgetary Institution of Science “Federal Scientific Center of Hygiene named after F.F. Erisman” of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
Author for correspondence.
Email: vostrikova.mv@fncg.ru
ORCID iD: 0000-0003-4090-5202
Junior Researcher at the Toxicology Department, Federal Scientific Centre of Hygiene named after F.F. Erisman of Federal Service for Supervision of Consumer Rights Protection and Human Wellbeing, Moscow region, Mytishchi, Russian Federation
e-mail: vostrikova.mv@fncg.ru
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