MODEL BIOMEMBRANES AS TEST OBJECTS FOR DETERMINING THE CONCENTRATION RANGE OF CHEMICALS WITHOUT DESTROING THE BIOLOGICAL OBJECTS

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Abstract

The paper presents data on changes in model biomembranes (liposomes, erythrocyte shadows, erythrocytes) used as test objects for determining those areas of concentrations of biologically active substances in which these is no violation of the structure or function of experimental objects. As biologically active substances synthetic ones were selected: plant growth regulator-melafen used in small doses in pre-sowing seed treatment and antioxidant derivatives -phenosan, phenoxan and Ihfans. It was shown by DSC method that phenosan derivatives in concentrations equal to 10-5 M and more destroy microdomain organization in bilayers of phospholipid multilamellar liposomes and reform protein microdomains in the shadows of erythrocytes. Melafen in small and large concentrations changes polymodally the microdomain organization in the bilayers of phospholipid multilamellar liposomes without destroying the structure and does not affect the protein microdomains in the shadows. Spectral analysis revealed an increase in membrane permeability in isolated whole erythrocytes under the action of melafen in large and small concentrations. The method of small-angle diffraction scattering showed the absence of melafen influence in a wide range of concentrations on the thickness of phospholipid bilayers and the order of their packing in multilamellar liposomes.

About the authors

O. M. Alekseeva

N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Author for correspondence.
Email: olgavek@yandex.ru
119334, Moscow Russian Federation

A. V. Krementsova

N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Email: akrementsova@mail.ru
119334, Moscow Russian Federation

A. V. Krivandin

N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Email: a.krivandin@sky.chph.ras.ru
119334, Moscow Russian Federation

O. V. Shatalova

N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Email: Shatalova@sky.chph.ras.ru
119334, Moscow Russian Federation

Yu. A. Kim

Institute of Cell Biophysics, Russian Academy of Sciences

Email: fake@neicon.ru
142290, Pushchino, Moscow region Russian Federation

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