Participation of jasmonic acid in the long-distance signalling from roots to shoots of peas plants under saliny

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Abstract

Jasmonic acid (JA) and its derivatives are involved in the adaptation of plants to biotic and abiotic stresses, including salinization. However, there is insufficient information about the role of JA in the transmission of signals from organ to organ under the local action of abiotic factors. The signaling role of JA in connection with the reaction of shoots to salinization of the root environment of pea plants has been studied. The results of the effect of salinization on changes in the content of JA in the growing and conducting zones of roots and xylem sap, as well as the localization of JA and abscisic acid (AA) in the leaves of stressed plants due to changes in their transpiration level are presented. The content and localization of JA in leaves and roots of plants were evaluated by immunohistochemical method using specific antibodies. The purpose of this study is to check whether salinity-induced changes in the concentration of JA in roots and xylem sap can explain the accumulation of these hormones in leaves and related transpiration changes.

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About the authors

G. R. Akhiyarova

Ufa Federal Research Center of the RAS

Author for correspondence.
Email: akhiyarova@rambler.ru

Ufa Institute of Biology

Russian Federation, prosp. October 69, Ufa 450054, Bashkortostan

G. Kh. Vafina

Ufa Federal Research Center of the RAS

Email: akhiyarova@rambler.ru

Ufa Institute of Biology

Russian Federation, prosp. October 69, Ufa 450054, Bashkortostan

A. V. Korobova

Ufa Federal Research Center of the RAS

Email: akhiyarova@rambler.ru

Ufa Institute of Biology

Russian Federation, prosp. October 69, Ufa 450054, Bashkortostan

I. I. Ivanov

Ufa Federal Research Center of the RAS

Email: akhiyarova@rambler.ru

Ufa Institute of Biology

Russian Federation, prosp. October 69, Ufa 450054, Bashkortostan

A. R. Giniyatullin

Ufa Federal Research Center of the RAS; Ufa University of Science and Technology

Email: akhiyarova@rambler.ru

Ufa Institute of Biology

Russian Federation, prosp. October 69, Ufa 450054, Bashkortostan; ul. Zaki Validi 32, Ufa 450076, Bashkortostan

E. R. Gaffarova

Ufa Federal Research Center of the RAS; Ufa University of Science and Technology

Email: akhiyarova@rambler.ru

Ufa Institute of Biology

Russian Federation, prosp. October 69, Ufa 450054, Bashkortostan; ul. Zaki Validi 32, Ufa 450076, Bashkortostan

M. I. Garipova

Ufa University of Science and Technology

Email: akhiyarova@rambler.ru
Russian Federation, ul. Zaki Validi 32, Ufa 450076, Bashkortostan

G. R. Kudoyarova

Ufa Federal Research Center of the RAS

Email: akhiyarova@rambler.ru

Ufa Institute of Biology

Russian Federation, prosp. October 69, Ufa 450054, Bashkortostan

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Supplementary files

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2. Fig. 1. Effect of salinity and shoot treatment with methyl jasmonate (+MJ, 10 mM) on the rate of water loss by 8-day-old go-root (Pisum sativum) plants (n 100). The time points after addition of sodium chloride (50 mM) to the nutrient solution were 1.5, 4.5 h, and 4 days. One and two asterisks denote averages significantly different from controls at p ≤0.05 and p ≤0.01, respectively (t-test).

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3. Fig. 2. Localization of ABA in leaf stomatal cells of 8-day-old control non-stressed pea (Pisum sativum) plants (a) and 1 h after salinization (b) of nutrient solution by sodium chloride (50 mM) application. The ruler corresponds to 5 µm.

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4. Fig. 3. Localization of jasmonic acid in leaf tissues and stomatal cells of 8-day-old control non-stressed pea (Pisum sativum) plants (a, c) and 4 h after salinization (b, d) of the nutrient solution by sodium chloride (50 mM) application. The ruler corresponds to 40 µm (a, b) and 5 µm (c, d); 1 - mesophyll, 2 - conductive bundle, 3 - stomata.

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5. Fig. 4. Localization of jasmonic acid on transverse sections of conductive tissues of the basal zone of the root at a distance of 5-7 mm from the shoot base of 8-day-old control unstressed plants (a) and after 1.5 h on the background of salinization caused by the addition of 50 mM sodium chloride (b) to the nutrient solution of pea plants. The ruler corresponds to 50 µm; xc, xylem, fl, phloem.

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6. Fig. 5. Localization of jasmonic acid on longitudinal sections of the meristematic zone of roots of 11-day-old control non-stressed plants (a) and after 4 days on the background of salinization caused by the addition of 50 mM sodium chloride (b) to the nutrient solution of pea plants.The ruler corresponds to 500 µm; 1 - root sheath, 2 - bark, 3 - central cylinder.

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