The effect of a new technical product of the chloroacetamide class on the parameters of acute oral toxicity and long-term effects on the body of rats

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Abstract

Introduction. The ability of pesticides to circulate in environmental objects (water, air, soil) and their presence in agricultural products cause the possibility of their chronic adverse effects on a living organism.

The aim of the study was to study the effect of a new technical product of the chloroacetamide class (TPH) on the parameters of acute oral toxicity (LD50) and long–term effects of action (embryotoxicity and teratogenicity), the establishment of a hazard class.

Material and methods. In accordance with the set goal in the biological testing laboratory (vivarium) of the FSUE “FNTSG named after F.F. Studies were conducted on the effect of a new technical product of the chloroacetamide class on the parameters of acute oral toxicity (LD50) in male rats, embryotoxicity and teratogenicity in pregnant rats. Hazard classes are established according to the methodological recommendations (MP No. 1.2.0235–21). Previously, studies in this volume have not been conducted.

Results. Results. It was found that the acute oral toxicity (LD50) of the studied compound is at the level of > 2000 mg/kg of body weight. According to the results of the long-term effect, it was revealed that the inactive dose (NOEL) for the mother was set at the level of 1/15 LD50 and NOEL for 1/5 LD50 fetuses (embryotoxicity, teratogenicity).

Limitations related to the analysis of the results of experimental data on the study of long-term effects (embryotoxicity and teratogenicity) of the pesticide without taking into account the carcinogenic effect on the body of warm-blooded (rats) as a result of exposure to a new technical product of the chloroacetamide class.

Conclusion. The studied technical product of the chloroacetamide class according to the hygienic classification of pesticides and agrochemicals by the degree of dangerity by acute oral toxicity (LD50) and by the long-term effects of action (embryotoxicity and teratogenicity) carried out on rodents (rats, males and females) refers to low-hazard compounds (hazard class 4).

Compliance with ethical standards. The study was approved by the Ethics Committee of the FBES "F.F. Erisman Federal Scientific Center of Hygiene of Rospotrebnadzor" (minutes of the meeting No. 11 of 12.10.2021).

Conflict of interest. The author declare no conflict of interest.

Funding. The study had no sponsorship.

Received: February 15, 2023 / Accepted: July 29, 2023 / Published: August 30, 2023

About the authors

Tatiana Mikhailovna Epishina

FBES «F.F. Erisman Federal Scientific Center of Hygiene of Rospotrebnadzor»

Author for correspondence.
Email: epishinatm@fferisman.ru
ORCID iD: 0000-0003-0331-0701

Doctor of Biological Sciences, Senior Researcher of the Department of Toxicology and Environmental Hygiene of the F.F. Erisman Federal Scientific Centre of Hygiene, Rospotrebnadzor, 141014, Mytischi, Moscow region, Russian Federation.

e-mail: epishinatm@fferisman.ru

Russian Federation

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