CONTENT OF METALLOTHIONEINS AND THE TOTAL PROTEIN IN SHEEP PERIPHERAL BLOOD LYMPHOCYTES UNDER CHRONIC DIETARY INTAKE OF LEAD NITRATE

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Abstract

The content of the total protein and metallothioneins in peripheral blood lymphocytes of sheep which received lead nitrate with the diet in concentration of 5;25 and 150 mg/kg fodder was examined. Blood samples were taken from the sheep jugular vein before feeding on 7, 14, 28, 42, 70 and 90 days of investigation. The chronic intake of lead with the diet by the sheep organism is characterized by an increased level of metallothioneins in peripheral blood lymphocytes with simultaneous decrease of animals viability. At the same time, the amount of the total protein in the initial period of intoxication raises and in the subsequent periods decreases. Based on results obtained, it is suggested to use the determination of the metallothioneins content in peripheral blood lymphocytes as informative parameter to estimate mammalians exposure to lead.

About the authors

Eldeniz Balabek ogly Mirzoev

State Scientific Institution» All-Russian Institute of Agricultural Radiology and Agroecology», Russian Academy of Agricultural Sciences

Author for correspondence.
Email: noemail@neicon.ru

Доктор биологических наук, ведущий научный сотрудник лаборатории радиобиологии и экотоксикологии сельскохозяйственных животных ГНУ ВНИИСХРАЭ, 249032, г. Обнинск, Калужская обл.,

e-mail: mirzoev.ed@yandex.ru

Russian Federation

Vladimir Olegovich Kobyalko

State Scientific Institution» All-Russian Institute of Agricultural Radiology and Agroecology», Russian Academy of Agricultural Sciences

Email: noemail@neicon.ru

Кандидат биологических наук, старший научный сотрудник лаборатории радиобиологии и экотоксикологии сельскохозяйственных животных ГНУ ВНИИСХРАЭ, 249032, г. Обнинск, Калужская обл.,

e-mail: kobyalko@yandex.ru

Russian Federation

Irina Vladimirovna Polyakova

State Scientific Institution» All-Russian Institute of Agricultural Radiology and Agroecology», Russian Academy of Agricultural Sciences

Email: noemail@neicon.ru

Младший научный сотрудник лаборатории радиобиологии и экотоксикологии сельскохозяйственных животных ГНУ ВНИИСХРАЭ, 249032, г. Обнинск, Калужская обл.

e-mail: irinaamchenkina@mail.ru

Russian Federation

Olga Aleksandrovna Gubina

State Scientific Institution» All-Russian Institute of Agricultural Radiology and Agroecology», Russian Academy of Agricultural Sciences

Email: noemail@neicon.ru

Научный сотрудник лаборатории радиобиологии и экотоксикологии сельскохозяйственных животных ГНУ ВНИИСХРАЭ, 249032, г. Обнинск, Калужской обл.

e-mail: Olgubina@yandex.ru

Russian Federation

Natalya Aleksandrovna Frolova

State Scientific Institution» All-Russian Institute of Agricultural Radiology and Agroecology», Russian Academy of Agricultural Sciences

Email: noemail@neicon.ru

Кандидат биологических наук, научный сотрудник лаборатории радиобиологии и экотоксикологии сельскохозяйственных животных ГНУ ВНИИСХРАЭ, 249032, г. Обнинск, Калужская обл.

e-mail: nafc@yandex.ru

Russian Federation

References

  1. Klaasen C.D., Liu J., Diwan B.A. Metallothionein protection of cadmium toxicity // Toxicol. Appl. Pharmacol., 2009; 238: (3): 101-112.
  2. Yu J., Fujishiro H., Oyama T.M., Hasegawa T., Seko Y., Miura N., Himeno S. Dichotomous effects of lead acetate on the expression of metallothionein in the liver and kidney of mice // Biol. Pharm. Bull., 2009; 32: 1037-1042.
  3. Pykhteeva E.G. The stude induction of metallothionein in the liver of mice under intraperitoneal introduction of divalent metals salts // Sovremennye problemy toksikologii pischevoy i himicheskoy bezopasnosti., 2012; 1: 20-24 (in Russian).
  4. Mirzoev E.B., Kobyalko V.O., Gubina O.A., Frolova NA The response reaction of organism of rats (generation F1 ) under the chronic exposure of cadmium in the antenatal and dairy period of feeding // Toxicological Review., 2011; 4: 16-19 (in Russian).
  5. Kobyalko V.O., Mirzoev E.B., Gubina O.A., Frolova N.A., Ratnikova L.I., Anisimov V.S. Content of metallothioneins in liver and kidneys of rats different age at intraabdominal administration of cadmium nitrate // Toxicological Review, 2011; 5: 18-21 (in Russian).
  6. Maitani T., Watahiki A., Suzuki K.T. Induction of metallothionein after lead administration by three injection routes in mice // Toxicol. Appl. Pharmacol, 1986; 83: 211-217.
  7. Wang L., Chen D.W., Wang H., Liu Z.P. Effects of lead and/or cadmium on the expression of metallothionein in the kidney of rats // Biol. Trace Elem. Res., 2009; 129: 190-199.
  8. Sungurov A. Yu. Separation and analise of cells by physical methods / Itogi nauki I tekhniki VINITI. Ser. «Tsitologiya», 1985; 4. (in Russian).
  9. Boyum A. Isolation of mononuclear cells and granulocytes from human blood // J. Clin. Invest. 1968; 21: 77-89.
  10. Eaton D.L., Toal B.F. Evaluation of the Cd/Hemoglobin affinity assay for the rapid determination of metallothionein in biological tissues // Toxicol. Appl. Pharmacol., 1982; 66:134-142.
  11. Koterov A.N., Trebenok ZA, Pushkareva N.B., Nikol’skii A.V. The effect of Zn-metallothionein on lipid peroxidation in rodent bone marrow cells // Radiatsionnaya biologiya. Radioekologiya, 1998;.38 (3): 426-430 (in Russian).
  12. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent // J. Biol. Chem., 1951; 193: 265-275.
  13. Lakin G.F. Biometriya: Uchebnoye posobiye dlya boil. spets.vusov biol. 4 izd., pererab. i dop. M.: Visshaya shkola. 1990 (in Russian).

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Copyright (c) 2014 Mirzoev E.B., Kobyalko V.O., Polyakova I.V., Gubina O.A., Frolova N.A.


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