COMPARATIVE EVALUATION OF THE EFFICACY OF PEPTIDE DRUGS AT SEVERE ACUTE POISOING WITH ETHANOL

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Abstract

A comparative assessment of efficacy of peptides Semax, Selank, KK-1, Bestim and Moleksan is presented at highly severe acute intoxication with ethanol, based on daily survival , neurological status, state of the organism certain vital functions. An extremely severe degree of alcoholic poisoning was modelled with intragastric administration of ethanol 40% solution at 12g/kg dose (1.5 LD50). Semax was administrated intra-nasally at dose of 3 mg/kg; Selank- intranasal dose of 3 mg/kg; KK-1 – intranasal dose of 40 μg/kg, Bestim – intranasal dose of 3 mg/kg; Moliksan intra-abdominal dose of 30 mg/kg. The preparations efficacy was evaluated by using the following application schemes: preventive one ( one- time an hour before ethanol administration); therapeutic and preventive ( an hour before and immediately after ethanol administration), early therapeutic ( immediately after ethanol administration and then once a day during 2 consecutive days) and postponed therapeutic (30 min. after ethanol administration and then once a day during two consecutive days). It was found out that in case of intoxication caused by 1,5 LD50 ethanol administration, preparation KK-1 was found to be the most efficacious under all schemes applied. The administration of this preparation prevented the nosogeny of coma and death of most poisoned animals. A preventive application of Moliksan significantly lowered the severity of poisoning., reduced 4.9-fold the number of animals in the state of terminal coma, increased by 66% survival in rats. Under the therapeutic and preventive application, Semax and Moliksan showed the greatest efficacy, providing 83% survival in poisoned rats. When applied for early therapeutic treatment, Moliksan appeared to be the most effective based on survival indicators and intoxication clinical picture. Under the postponed scheme of application, the therapeutic action of peptides was low effective.

About the authors

Aleksandr Nikolaevich Grebenyuk

Federal State Budgetary Military Educational Establishment of Higher Professional Education «S.M. Kirov Military Medical Academy», Ministry of Defense of the Russian Federation

Author for correspondence.
Email: noemail@neicon.ru

Доктор медицинских наук, профессор, профессор кафедры военной токсикологии и медицинской защиты Военно-медицинской академии им. С.М. Кирова МО РФ, 194044, г. Санкт-Петербург

e-mail: grebenyuk_an@mail.ru

Russian Federation

Denis Aleksandrovich Halyutin

Federal State Budgetary Military Educational Establishment of Higher Professional Education «S.M. Kirov Military Medical Academy», Ministry of Defense of the Russian Federation

Email: noemail@neicon.ru

Адъюнкт при кафедре военной токсикологии и медицинской защиты Военномедицинской академии им. С.М. Кирова МО РФ, 194044, г. Санкт-Петербург

e-mail: hal-denis81@yandex.ru

Russian Federation

Vladimir Leonidovich Reynyuk

Federal State Budgetary Military Educational Establishment of Higher Professional Education «S.M. Kirov Military Medical Academy», Ministry of Defense of the Russian Federation

Email: noemail@neicon.ru

Доктор медицинских наук, доцент кафедры военной токсикологии и медицинской защиты Военно-медицинской академии им. С.М. Кирова МО РФ, 194044, г. Санкт-Петербург

e-mail: vladton@mail.ru

Russian Federation

Aleksandr Aleksandrovich Kolobov

Federal State Unitary Establishment «State Research Institute of Highly Pure Bio Preparations» Federal Medical and Biological Agency of Russia

Email: noemail@neicon.ru

Доктор биологических наук, заведующий лабораторией химии пептидов Государственного научно-исследовательского института особо чистых биопрепаратов ФМБА России, 197170, г. Санкт-Петербург

kolobov@hpb-spb.com

Russian Federation

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Copyright (c) 2014 Grebenyuk A.N., Halyutin D.A., Reynyuk V.L., Kolobov A.A.


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