Features of disturbance of oxidative stress markers and venous blood cells apoptosis in the early stage of acute poisoning by corrosive substances

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Abstract

The character of disturbance of oxidative stress markers and venous blood cells apoptosis in patients with corrosive substances poisoning in early stage was studied. In the first 5 days for the estimation of the oxidative stress severity the lipid peroxidation (LPO) products, antioxidant system (AOS) state and value of the platinum electrode open circuit potential (OCP) were determined. Apoptosis was studied by measuring of apoptotic lymphocytes and dead leukocytes concentration. It was found that in patients younger than 60 years in response to the intake of corrosive substance, peroxidation processes are the first to activate, as evidenced by the oxidative stress development and its increase in dynamics. The apoptosis processes are activated only as oxidative stress severity increase which indicates the development of an adequate reaction to the acute chemical trauma in persons of working age. Contrariwise, in patients older than 60 years apoptosis of venous blood lymphocytes is first activated followed by peroxidation processes activation. Moreover, the low activity of LPO/AOS system is noted in the whole. This fact indicates the absence of an adequate reaction to the intake of corrosive substance in this category of patients.

About the authors

K. K. Il′yashenko

Sklifosovsky Research Institute for Emergency Medicine, Public Healthcare Institution of Moscow Healthcare Department

Author for correspondence.
Email: toxikapa@mail.ru

Il’yashenko Kapitalina Konstantinovna

129090, Moscow

Russian Federation

A. K. Evseev

Sklifosovsky Research Institute for Emergency Medicine, Public Healthcare Institution of Moscow Healthcare Department

Email: anatolevseev@gmail.com

Evseev Anatoly Konstantinovich

129090, Moscow

Russian Federation

N. V. Borovkova

Sklifosovsky Research Institute for Emergency Medicine, Public Healthcare Institution of Moscow Healthcare Department

Email: borovkovanv@yandex.ru

Borovkova Natal’ya Valer’evna

129090, Moscow

Russian Federation

E. V. Klychnikova

Sklifosovsky Research Institute for Emergency Medicine, Public Healthcare Institution of Moscow Healthcare Department

Email: klychnikovaev@mail.ru

Klychnikova Elena Valer’evna

129090, Moscow

Russian Federation

I. V. Goroncharovskaya

Sklifosovsky Research Institute for Emergency Medicine, Public Healthcare Institution of Moscow Healthcare Department

Email: goririna22@gmail.com

Goroncharovskaya Irina Viktorovna

129090, Moscow

Russian Federation

A. Yu. Simonova

Sklifosovsky Research Institute for Emergency Medicine, Public Healthcare Institution of Moscow Healthcare Department

Email: simonovatoxy@mail.ru

Simonova Anastasiya Yur’evna

129090, Moscow

Russian Federation

E. V. Tazina

Sklifosovsky Research Institute for Emergency Medicine, Public Healthcare Institution of Moscow Healthcare Department

Email: TazinaEV@sklif.mos.ru

Tazina Elizaveta Vladimirovna

129090, Moscow

Russian Federation

A. N. El′kov

Sklifosovsky Research Institute for Emergency Medicine, Public Healthcare Institution of Moscow Healthcare Department

Email: 2bobika@mail.ru

El’kov Aleksander Nikаnorovich

129090, Moscow

Russian Federation

M. M. Potskhveriya

Sklifosovsky Research Institute for Emergency Medicine, Public Healthcare Institution of Moscow Healthcare Department

Email: potskhveriya@mail.ru

Potskhveriya Mikhail Mikhailovich

129090, Moscow

Russian Federation

A. K. Shabanov

Sklifosovsky Research Institute for Emergency Medicine, Public Healthcare Institution of Moscow Healthcare Department

Email: aslan_s@mail.ru

Shabanov Aslan Kurbanovich

129090, Moscow

Russian Federation

Yu. V. Andreev

Sklifosovsky Research Institute for Emergency Medicine, Public Healthcare Institution of Moscow Healthcare Department

Email: AndreevYV@sklif.mos.ru

Andreev Yuliy Vadimovich

129090, Moscow

Russian Federation

References

  1. Ostapenko Y.N., Kovalev A.V., Gasimova Z.M., Zaikovskiy V.V. Toxicological aid to population of the Russian Federation: status and problems. Toxicological Review. 2014; (3): 2-8 (in Russian).
  2. Data from the Federal State Statistics Service. The incidence of the population according to the main disease classes. Available at: http://www.gks.ru/free_doc/new_site/population/zdrav/zdr2-1.xls (accessed 25 may 2020).
  3. Gummin D.D., Mowry J.B., Spyker D.A., Brooks D.E., Osterthaler K.M., Banner W. 2017 Annual Report of the American Association of Poison Control Centers’ National Poison Data System (NPDS): 35th Annual Report. Clinical toxicology. 2018; 56(12): 1213-1415.
  4. Luzhnikov E.A., ed. Medical toxicology. Moscow: GEOTAR Media; 2012 (in Russian).
  5. Orlov Y.P., Orlova N.V., Mikheev E.Y., Beneskriptov I.S. Acetic acid poisoning. A new look at the old “Russian disease” problem. Omsk: Taktik-Studio; 2015 (in Russian).
  6. Shikalova I.A., Lodyagin A.N., Barsukova I.M., Nasibullina A.R., Kalloyda D.Y. The analysis of toxicological situation according to three specialized centers of Russian Federation. Russian Sklifosovsky Journal “Emergency Medical Care”. 2019; 8(4):373-378 (in Russian).
  7. Prokhorovskaya A.G., Kuvakova R.I., Mokrushin A.V., Ilyashenko K.K., Sukhodolova G.N. Federal clinical guidelines. Toxic effect of corrosive substances. Toxic effects of soaps and detergents. Moscow; 2014 (in Russian).
  8. Luzhnikov E.A. Clinical toxicology. Moscow: Meditsina; 1999 (in Russian).
  9. Klodchenko N.P. Hematologic and coagulation disorders in case of acetic acid poisoning. Clinical Medicine. 1973; 51(9): 123-127 (in Russian).
  10. Belova M.V., Ilyashenko K.K., Luzhnikov E.A., Nimaev Z.T., Pinchuk T.P. Oxidative stress in acute poisoning by cauterant agents. Toxicological Review. 2007; (6): 33-37 (in Russian).
  11. Tsarkova T.G., Evseev A.K., Potskhveriya M.M., Simonova A.Yu. Blood plasma open circuit potential monitoring in patients with acute poisoning by cauterants. Advances in chemistry and chemical technology. 2017; 31(6): 8-10 (in Russian).
  12. Ilyashenko K.K., Belova M.V., Simonova A.Y., Borovkova N.V., Andreyev Y.V., Potskhveriya M.M. Apoptosis of blood cells in geriatric patients with psychopharmacological drug. Russian Sklifosovsky Journal “Emergency Medical Care”. 2017; 6(3): 210-215 (in Russian).
  13. Gavrilov V.B., Gavrilova A.R., Mazhul’ L.M. Methods of determining lipid peroxidation products in the serum using a thiobarbituric acid test. Questions of medical chemistry. 1987; 33 (1): 118-122 (in Russian).
  14. Khubutiya M.Sh., Evseev A.K., Kolesnikov V.A., Goldin M.M., Davydov A.D., Volkov A.G. et al. Measurements of platinum electrode potential in blood and blood plasma and serum. Electrochemistry. 2010; 46 (5): 569-573 (in Russian).
  15. Golikov P.P., Nikolaeva N.Yu. Analysis of nitrites/nitrates (NOx) in serum. Biomedical chemistry. 2004; 50(1): 79-85 (in Russian).
  16. Kalinina V.N., Soloviev V.I. Introduction to multivariate statistical analysis. Tutorial. Moscow; 2003 (in Russian).
  17. Belova M.V., Ilyashenko K.K., Luzhnikov Y.A. Oxidative stress in emergency toxicology. General Reanimatology. 2009; 5(6): 40-45 (in Russian).
  18. Klychnikova E.V., Tazina E.V., Smirnov S.V., Spiridonova T.G., Zhirkova E.A., Borisov V.S. et al. Correlation between biochemical parameters of oxidative stress, endogenous intoxication and regulation of vascular tone in patients with burn injury. Anesthesiology and resuscitation. 2015; (1): 45-49 (in Russian).
  19. Potskhveriya М.М., Evseev A.K., Simonova A.Y., Klychnikova E.V., Goroncharovskaya I.V., Matkevich V.A. et al. Interrelation of platinum electrode open circuit potential in the blood plasma and oxidative stress coefficient in patients with acute poisoning by cauterant agents. Toxicological Review. 2018; (2): 7-12 (in Russian).
  20. Bendich A., D’Apolito P., Gabriel E., Machlin I.J. Modulation of the immune system function of guinea pigs by dietary vitamin E and C following exposure to oxygen. Federation Proceedings. 1983; 42(4): 923.
  21. Halliwell B., Gutteridge J.M.C. Lipid peroxidation, oxygen radicals, cell damage, and antioxidant therapy. Lancet. 1984; 323(8391): 1396-1397.
  22. Bobyrev V.N., Pochernyaeva V.F., Starodubtsev S.G., Bobyreva L.E., Dubinskaya G.M., Voskresensky O.N. The specificity of the antioxidant protection systems of the organs and tissues - a basis for the differentiated pharmacotherapy with antioxidants. Experimental and clinical pharmacology. 1994; 57(1): 47-54 (in Russian).
  23. Klebanov G.I., Teselkin Yu.O., Baben- kova I.V., Lyubitsky O.B., Vladimirov Yu.A. Serum antioxidative activity. Vestnik RAMN. 1999; (2): 15-22 (in Russian).
  24. Vilmaz T., Kogan E.G. The role of oxidants and antioxidants in adenoid hyper- trophy in children. International journal of pediatric otorhinolaryngology. 2004; 68(8): 1053-1058.
  25. Anderson R., Lukey P.T., Theron A.J., Dippenaar U. Ascorbate and cysteine-mediated selective neutralisation of extracellular oxidants during N-formyl peptide activation of human phagocytes. Agents Actions. 1987; 20(1-2): 77-86.
  26. Menshchikova E.B., Lankin V.Z., Zenkov N.K., Bondar’ I.A., Krugovykh N.F., Trufakin V.A. Oxidative stress. Prooxidants and antioxidants. Moscow: Slovo; 2006. (in Russian).
  27. Skulachev V.P. Bioenergetic aspects of apoptosis, necrosis and mitoptosis. Apoptosis. 2006; 11 (4):473-485.

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Copyright (c) 2021 Il′yashenko K.K., Evseev A.K., Borovkova N.V., Klychnikova E.V., Goroncharovskaya I.V., Simonova A.Y., Tazina E.V., El′kov A.N., Potskhveriya M.M., Shabanov A.K., Andreev Y.V.


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