NEW DATA ON THE QUESTION OF INFORMATIVENESS OF EXPERIMENTS ON CELL CULTURES FOR ASSESSMENT OF COMPARATIVE AND COMBINED TOXICITY OF METAL OXIDE NANOPARTICLES

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Abstract

Isolated and combined damaging effects of PbO and CuO nanoparticles have been estimated on an established line of human fibroblasts using three different cytotoxicity indices based on reduction in: (a) the cellular dehydrogenase activity (MTT Assay), (b) the ATP content (Luminescent Cell Viability Assay), (c) the cellular proliferation, viability, spreading, and attachment to substrate evaluated integrally by continuous impedance-based measurement of the Normalized Cell Index. For all these indicators a clear dependence of cell damage on concentration of metal oxide nanoparticles has been demonstrated for both types of metal oxide nanoparticles, which is adequately described by the hyperbolic function, while at the same level of exposure the quantitative characteristics of cytotoxicity of PbO-NPs in comparison with CuO-NPs are similar. The latter was previously observed in the subchronic experiment on rats. The combined in vitro cytotoxicity of nanoparticles has been also described mathematically using the response surface construction methodology and found to be ambiguous, which is also consistent with the conclusions from the experiment on rats with the same nanoparticles.

About the authors

T. V. Bushueva

Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Author for correspondence.
Email: bushueva@ymrc.ru

Bushueva Tatiana Victorovna

620014, Ekaterinburg

Russian Federation

I. A. Minigalieva

Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: ilzira-minigalieva@yandex.ru

Minigalieva Ilzira Amirovna

620014, Ekaterinburg

Russian Federation

V. G. Panov

Institute of Industrial Ecology, Urals Branch of the Russian Academy of Sciences

Email: vpanov@ecko.uran.ru

 

Panov Vladimir Grigorievich

620990, Ekaterinburg

Russian Federation

A. N. Kuznetsova

Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: alisa@ymrc.ru

Kuznetsova Alisa Nikolaevna

620014, Ekaterinburg

Russian Federation

A. S. Naumova

Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: naumova@ymrc.ru

Naumova Anna Sergeevna

620014, Ekaterinburg

Russian Federation

M. P. Sutunkova

Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: sutunkova@ymrc.ru

Sutunkova Marina Petrovna

620014, Ekaterinburg

Russian Federation

V. Ya. Shur

School of Natural Sciences and Mathematics, Ural Federal University

Email: vladimir.shur@urfu.ru

Shur Vladimir Yakovlevich

620000, Ekaterinburg

Russian Federation

Е. V. Shishkina

School of Natural Sciences and Mathematics, Ural Federal University

Email: ekaterina.shishkina@labfer.usu.ru

Shishkina Ekaterina Vladimirovna

620000, Ekaterinburg

Russian Federation

V. B. Gurviсh

Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: gurvich@ymrc.ru

Gurvich Vladimir Borisovich

620014, Ekaterinburg

Russian Federation

B. A. Katsnelson

Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: bkaznelson@etel.ru

Katsnelson Boris Aleksandrovich

620014, Ekaterinburg

Russian Federation

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Copyright (c) 2019 Bushueva T.V., Minigalieva I.A., Panov V.G., Kuznetsova A.N., Naumova A.S., Sutunkova M.P., Shur V.Y., Shishkina Е.V., Gurviсh V.B., Katsnelson B.A.


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