Some aspects of metal oxide nanoparticles toxicity assessment on cell cultures as exemplified by NiO and Mn3O4

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Abstract

Comparative and combined damaging actions of NiO and Mn3O4 anoparticles were estimated on cultures of different established human cell lines. It was found out that the addition of the fetal bovine serum (FBS) to the culture media ,used in the investigation, renders NiO-NPs and, to even a greater extent, Mn3O4-NPs exponentially soluble while without FBS their dissolution was extremely low. Along with it, sedimentation of those MeO-NPs caused by their aggregation noticeably slowed down in the presence of the same FBS. The dependence of cell damage on the MeO-NPs concentration was found out, at a higher cytotoxicity of Mn3O4-NP as compared to NiO-NP. Thus, comparative assessment of NPs non-specific toxicity previously obtained in animal experiments was reproduced in the «in vitro» tests. However, with respect to manganese-specific brain damage «in vivo» discovered previously in sub-chronic intoxication with the same MeO-NPs, the present «in vitro» experiment on neurons only showed a certain enhancing effect of Mn3O4-NP on the action of NiO-NP, but the role of NiO-NP in the combination prevailed.

About the authors

I. А. Minigalieva

Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Author for correspondence.
Email: noemail@neicon.ru
Russian Federation

T. V. Bushueva

Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: noemail@neicon.ru
Russian Federation

V. G. Panov

Institute of Industrial Ecology, the Urals Branch of the Russian Academy of Sciences

Email: noemail@neicon.ru
Russian Federation

A. N. Varaksin

Institute of Industrial Ecology, the Urals Branch of the Russian Academy of Sciences

Email: noemail@neicon.ru
Russian Federation

V. Ya. Shur

School of Natural Sciences and Mathematics, the Ural Federal University

Email: noemail@neicon.ru
Russian Federation

E. V. Shishkina

School of Natural Sciences and Mathematics, the Ural Federal University

Email: noemail@neicon.ru
Russian Federation

V. B. Gurviсh

Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: noemail@neicon.ru
Russian Federation

B. A. Katsnelson

Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: noemail@neicon.ru
Russian Federation

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Copyright (c) 2017 Minigalieva I.А., Bushueva T.V., Panov V.G., Varaksin A.N., Shur V.Y., Shishkina E.V., Gurviсh V.B., Katsnelson B.A.


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