EXPOSURE TO VINYL CHLORIDE METABOLITES STUDIED IN WHITE RATS

The paper presents experimental data on the effect of vinyl chloride metabolites on the white rats behavior and results of their quantitative content in biological fluids of animals exposed to xenobiotics . Animals divided into groups received intraperitoneal injections of chloroethanol (CE) in a concentration of 20 mg/kg, monochloroacetic acid (MСAA) in a concentration of 10 mg/kg, control animals received distilled water. A total duration of exposure to xenobiotics was 2 weeks. The animals were surveyed in the tests “maze” and “open field”. To measure the concentrations of metabolites in biological samples, were used gas chromatography techniques with electron capture (GS-ECD) and mass selective detection (GC-MS). It was found out that the exposure to chloroethanol and monochloroacetic acid affected the behavior of experimental animals. Exposure to cloroethanol contributed to the development of anxiety and increased level of exploratory activity in experimental animals. Intoxication with monochloroacetic acid also contributed to a more pronounced exploratory behavior along with excitment (agitation). In animals exposed to CE, the presence of this compound in blood was revealed an hour after termination of the last injection. However as early as 2 hours after exposure, the concentration of CE decreased 13-fold. In control animals chloroethanol in the blood was not found. In two experimental groups as well as in the control, the presence of MCAA was not detected. The content of thyodiacetic acid (TDAA) significantly differed from the control values.

experimental studies, vinyl chloride, vinyl chloride metabolites, gas chromatography, behavior of white rats