Challenges and Features of Polysaccharide Visualization in Sorbent Layers During Planar Chromatography

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The features of planar (thin-layer) chromatography of several linear non-ionic polysaccharides (amylose, pullulan), their visualization by post-chromatographic derivatization with iodine, and the factors influencing this process are described. It is shown that the coloration of pullulan results from the formation of pseudoclathrate complexes of molecular iodine–iodide–pullulan in short helical segments of the polysaccharide, as well as iodine absorption in the bends of the polymer chain. It was established that the shift of the spectral absorption maximum of amylose (λmax ~ 600 nm, blue coloration) to the shorter wavelength region (λmax = 560 nm, red-brown coloration) is associated with specific interactions of starch with silica and the formation of a molecular iodine–iodide-anion–amylose–silica gel complex. It was demonstrated that the intensity of iodine staining of high-molecular-weight polysaccharides does not depend on their degree of polymerization, and polysaccharide visualization by the iodine reaction in thin-layer chromatography is possible only on neutral forms of silica sorbents.

作者简介

I. Malakhova

Branch of Petersburg Nuclear Physics Institute named by B.P.Konstantinov of National Research Centre «Kurchatov Institute» – Institute of Macromolecular Compounds

Email: a.kirillov2622@gmail.com
St. Petersburg, 199904, Russia

A. Kirillov

Branch of Petersburg Nuclear Physics Institute named by B.P.Konstantinov of National Research Centre «Kurchatov Institute» – Institute of Macromolecular Compounds

Email: a.kirillov2622@gmail.com
St. Petersburg, 199904, Russia

N. Gorshkov

Branch of Petersburg Nuclear Physics Institute named by B.P.Konstantinov of National Research Centre «Kurchatov Institute» – Institute of Macromolecular Compounds

Email: a.kirillov2622@gmail.com
St. Petersburg, 199904, Russia

A. Eremin

Branch of Petersburg Nuclear Physics Institute named by B.P.Konstantinov of National Research Centre «Kurchatov Institute» – Institute of Macromolecular Compounds

Email: a.kirillov2622@gmail.com
St. Petersburg, 199904, Russia

V. Krasikov

Branch of Petersburg Nuclear Physics Institute named by B.P.Konstantinov of National Research Centre «Kurchatov Institute» – Institute of Macromolecular Compounds

编辑信件的主要联系方式.
Email: a.kirillov2622@gmail.com
St. Petersburg, 199904, Russia

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