Vol 100, No 3 (2021)

Cover Page

Articles

Title page and table of contents of the Journal

Editorial Р.

Abstract

Title page and table of contents of the Journal

Hygiene and Sanitation. 2021;100(3):
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ENVIRONMENTAL HYGIENE

Assessment of carcinogenic risk caused by the impact of the environmental factors on urban population health

Rakitskii V.N., Stepkin Y.I., Klepikov O.V., Kurolap S.A.

Abstract

Introduction. The high level of environmental pollution in industrial cities, including carcinogens, causes an unacceptable risk to public health. In this regard, a quantitative risk assessment is required to develop preventive measures to reduce it.

The aim of the study was to assess the carcinogenic risk to the health of the population of an industrialized city, caused by the probable presence of carcinogens in the ambient air, drinking water of the centralized water supply system, and the soil of the residential area.

Material and research methods. The laboratory control of the content of carcinogens in environmental objects of the city of Voronezh for 2017-2020 was used as the reference data. The carcinogenic risk was assessed under the provisions of Guideline R. 2.1.10.1920-04 “Guidelines for assessing the risk to public health when exposed to chemicals that pollute the environment.”

Results. Taking into account the regional peculiarities of the composition of pollutant emissions into the atmospheric air, 1,3-butadiene (source - production of synthetic rubber) should be classified as priority carcinogens requiring systematic monitoring and measures to reduce emissions and, accordingly, concentrations in the surface layer of atmospheric air and chromium6+ compounds (the main source is an aircraft plant), which contribute 69.9-75.7% and 21.7-26.9%, respectively, to the total values ​​of the individual carcinogenic risk, which exceeds the maximum permissible level (1 • 10–4). The carcinogenic risk from exposure to drinking water pollutants (halogenated organic substances) and soil (under the scenario of accidental ingestion by preschool children) is below the maximum permissible risk (1 • 10–4).

Conclusion. It is necessary to pay attention to increasing the sensitivity of the applied laboratory control methods, expanding the list of controlled carcinogenic pollutants, improving the monitoring system of carcinogens, and implementing measures to reduce the carcinogenic risk associated with atmospheric air pollution.

Hygiene and Sanitation. 2021;100(3):188-195
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Estimation of chemical safety of environmental protection technologies for atmosphere pollution reduction (a case study of processess of laser treatment of polymer materials)

Malysheva A.G.

Abstract

Introduction. One way to protect atmospheric air from industrial pollution is the use of environmental protection technologies aimed at emissions purification. As a result of the man-induced impact, the industrial emissions can change the composition of air medium, while the resulting products of physical and chemical transformations with other, sometimes more toxic properties, can influence on the quality and chemical safety of atmospheric air in comparison with the initial substances entering it as part of industrial emissions.

Materials and methods. Studies of the impact of various technologies of treatment the industrial atmospheric emissions of organic glass and polymer materials processing plants with laser systems were carried out using a chromato-mass spectrometric system Focus GC с DSQ II (USA).

Results. We have established the change in the qualitative-quantitative composition of emissions from organic glass laser treatment processes using various cleaning devices. Estimation of electrostatic purification technology efficiency based on plasma-catalytic oxidation with atomic oxygen revealed an increase in the transformation products - aldehydes. The cleaning devices seems ineffective and chemically unsafe concerning one of the primary pollutants included in the emissions of organic glass and polymer materials processing plants with laser systems - methyl methacrylate.

Conclusion. The use of new environmental protection technologies for emissions purification can cause a change in their qualitative-quantitative composition, which requires chemical-analytical control. It is advisable to use chromatography-mass spectrometry to identify and quantify up to 100 substances simultaneously in the air with an unknown composition of pollutants to assess the efficiency and chemical safety of new technologies for emissions purification adequately. It will certainly reduce and minimize health risks and allow considering the possibility of man-induced formation of transformation by-products.

Hygiene and Sanitation. 2021;100(3):196-203
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Halotolerance of pseudomonads isolated from aquatic environment and fish (Sander lucioperca) in the Volga River delta

Obukhova O.V., Lartseva L.V.

Abstract

Introduction. The material shows halophiles bacteria R. Psendomonas the water’s microbiota and the pikeperch in spring and autumn. The attachment of Pseudomonas isolated from these habitats, certain Delta areas has not been identified. 

Materials and methods. 190 “water” strains and 720 ones isolated from fish were experimentally tested. In meat-peptone broth (MPB) with 3.0, 7.0, and 10.0% NaCl content, daily cultures of analyzed bacteria were sown, incubation of which was carried out at 37 °C, and the results were taken into account after 24 and 48 hours.

Results. There were no significant differences in halophilicity in the analyzed strains, except for the autumn season, especially in 10.0% salt solution. Among the isolated pseudomonads, the maximum halotolerance in both biotopes was observed in P. fluorescens and P. alcaligenes in P. putida.

Halophiles strains of Pseudomonas that infect the water and fish had seasonal cycles. A slight decrease in the halophilicity of pseudomonades persistent in water and fish only at concentrations of 3.0 and 10.0 mg/l from spring to summer (1.1-1.2 times), and their significant rise in autumn (1.5 and 1.4 times) in the salt concentration of 3.0 mg/l. In the tested strains in spring and autumn, increased salt tolerance values were noted, which was determined by the hydrological and hydrochemical features of Delta waters and the “transfer” of bacteria in the body of walleye during its migration from the sea to the river.

Conclusion. Analysis of long-term material showed high halophiles studied strains of pseudomonad, indicating that their sanitary and epidemiologic danger, and the ability to remain viable in salted fish products

Hygiene and Sanitation. 2021;100(3):204-207
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Heavy metals and the "Chernobyl trace" in the bottom sediments of the internal reservoir: The Gulf of Kaliningrad

Bogdanov N.A., Bass O.V., Savostina O.A., Yermolayev B.V., Lyapounov S.M.

Abstract

Introduction. The article contains the results of a study of environmental hazards and threats to public health from the accumulation of heavy metals (HM) and the activity of man-made and natural radionuclides (NRN) in the bottom sediments of the inner reservoir r – the Kaliningrad Bay at the turn of the XX-XXI centuries (1986-2019). The Consumption of polluted resources (seafood, bottom sediments as fertilizers, and mineral raw materials) can negatively affect residents’ health of not only this region. The problems are considered: 1) the mechanism and intensity of the long-term accumulation of Hg and other HMs, 2) the activity of the 137Cs isotope (“Chernobyl trace”) and NRN in comparison with the radiation state of the beach Sands in Latvia, 3) the biomedical threats from the factors considered.

Results. The water’s closed area led to the role of the bay as a giant trap of sedimentary material and pollutants. Hg in the water reached dangerous concentrations of 0.15-0.6 mcg/l (MPC = 0.5 mcg/l). The accumulation of Hg in the bottom sediments of the Gulf estuary and Primorskaya Bay has reached a low level of pollution over 20 years (Norwegian criteria: from 50-90 to 155-252 mcg/kg) and has approached the safe limit – the target level (300 mcg/kg – the national, regional standard and Dutch lists of the pollutants). The Hg content also increased in the background (from 23 to 82 mcg/kg). The most dangerous foci of sanitary and toxicological hazard (Zc-t index (AgPbCoW) >6, up to 13) coincide with the borders of dangerous accumulation of the sum of HM (Zc(MnCrVNiCoAgZnPbSnW) >34, up to 61) and Hg (>100-150 mcg/kg). The initial activity of 137Cs in the Baltics decreased, Bq/kg: from 110 (beaches of Latvia) to 104 and 99 (bottom sediments; coasts of Lithuania, Kaliningrad region, and Poland). The specific activity of NRN in the bottom sediments of the Gulf (510-572 Bq/kg) is below the safe level (740 Bq/kg); handling them has no restrictions.

Conclusions. An increase in the amount of Hg causes the danger. The trend requires research and monitoring, including Hg in seafood and biomonitoring.

Hygiene and Sanitation. 2021;100(3):208-217
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The role of environmental factors in the realization of the epidemic process on ascariasis

Martsev A.A.

Abstract

Introduction. Ascariasis is one of the most common parasitic diseases that infect about 1.5 million people in the world every year. In Russia, from 40 to 60 thousand cases are registered annually. To make effective management decisions on sanitary-epidemiological and preventive measures, the search for environmental factors that potentially affect the epidemic process of ascariasis in the Vladimir region was carried out.

Materials and methods. The study analyzed archived statistical data on the incidence of ascariasis in the population, the state of the environment (air, water, and soil pollution), the socio-economic situation (unemployment rate, average salary, number of doctors and nurses, housing provision), as well as climate indices (average monthly temperature, number of days in a month with precipitation, humidity and snow cover) in the region. Statistical data processing and correlation and regression analysis were performed using the Statistica software. The maps were built and edited using the ArcView 3.1 GIS program and the standard Paint computer program.

Results. The incidence of ascariasis in the region is characterized by significant diversity. Statistically reliable correlations of morbidity with environmental indices were obtained, and a mathematical equation was constructed using linear regression to predict the level of morbidity in the region.

Conclusion. In the epidemiology of ascariasis in the Vladimir region, a leading role retains climatic, environmental indices determining the possibility of developing eggs of ascarids in the environment to the infective stage. A regional factor that affects the epidemiological process (indirectly through the suppression of the protective functions of the child’s body and reducing the development time of Ascaris eggs) is air pollution by stationary objects. The data obtained can assess the risk of infection with ascariasis to ensure biological safety in the region.

Hygiene and Sanitation. 2021;100(3):218-222
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Environmental factors and cardiovascular diseases

Chaulin A.M., Duplyakov D.V.

Abstract

Introduction. New advances in the diagnosis and treatment of cardiovascular diseases (CVD), as practice shows, are not able to significantly improve the statistical indicators of morbidity and mortality of CVD. This fact indicates that there are additional factors and mechanisms that are important to consider, both for prevention and for the most optimal management of patients. Recently, the relationship between environmental and lifestyle factors with CVD has been actively studied. However, despite understanding the relationship between environmental factors and various diseases, including CVD, the mechanisms by which specific factors increase or decrease the risk of developing CVD are not yet fully understood, and a number of studies are contradictory.

The aim of our work was to generalize existing data on the impact of such critical environmental factors as air pollution and solar insolation on the cardiovascular system, as well as to comprehensively discuss the mechanisms by which these environmental factors can participate in the development and progression of CVD. To achieve our work’s goal, we analyzed modern foreign literature using the PubMed database.

Conclusion. According to numerous experimental and clinical studies, air pollution and solar insolation deficiency play an essential role in developing CVD and the aggravation of patients with various CVD (atherosclerosis, hypertension, coronary heart disease, heart failure, myocardial infarction, and stroke). Thus, air pollution and lack of solar insolation can be considered as critical risk factors for CVD. Future research should focus on the study and establishment of specific pathogenetic mechanisms by which environmental factors affect the cardiovascular system’s health to develop effective treatment and prevention measures.

Hygiene and Sanitation. 2021;100(3):223-228
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OCCUPATIONAL HEALTH

Comparative physiological and hygienic assessment of the personal protective equipment EP-4(0) in the various assembly according to human thermal state indices

Burmistrova O.V., Perov S.Y., Konshina T.A.

Abstract

Introduction. The article presents results of the study of the impact of the personal protective equipment EP-4 (0) in various assembly (overalls, jacket and trousers, jacket and semi-overall) from power frequency electric field and electrical shock on the thermal state of workers in a heating environment at air temperature 34.8 °C and relative humidity 48%.

Materials and methods. The study involved three men aged 35.3±4.6 years. They did the physical exercise for 40 minutes, had comfortable relaxation for 30 minutes after work. Recorded indices included skin temperature and moisture sensation score on 11 parts of the body, body temperature in the ear canal, heart rate, clothes temperature, heat sensation score, moisture loss, moisture evaporation efficiency.

Results. Overalls using had the highest values of heat content, moisture score, heart rate changes, and body temperature increment. Heat content index in the body reached maximum permissible value using overalls earlier than other configurations.

Conclusion. The research data showed overalls have the most significant impact on the human thermal state, jacket and trousers have the least impact, jacket and semi-overall take an intermediate place. The configuration including jacket and trousers, jacket and semi-overall under specific operating conditions is the most optimal. Such PPE assembly can be recommended for use in the open area in the summer season at an air temperature of 35 °C and above, subject to protective requirements.

Hygiene and Sanitation. 2021;100(3):229-233
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The intestinal microbiota of rats under the influence of biotechnological strains of microorganisms from various taxonomic groups

Sheina N.I., Budanova E.V., Kolesnikova V.V., Mjalina L.I., Sazonova L.I.

Abstract

Introduction. The microbiota of the gastrointestinal tract (GIT) plays an essential role in maintaining human health. Many factors, including industrial pollutions with biotechnological strains of microbes, can affect the normal balance of intestinal microbiota. The biotechnological industry nowadays produces a wide range of products for medical and veterinary use, agriculture, food, chemical industries, etc. To develop hygienic standards that regulate the possible adverse effect of biotechnological strains of microorganisms on workers’ health, the intestinal microflora of rats in the experiment can be studied. The data obtained were used as the basic concept in elaborating state sanitary standards for limitations of the concentrations of biotechnological strains of microorganisms in the ambient air of the working area and settlements’ atmosphere.

Materials and methods. We have tested 52 strains of microorganisms applied in biotechnology as producers of a variety of biological substances. They included members of different taxonomic groups: gram-positive and gram-negative bacteria, actinomycetes, molds, and yeasts. The experiments were carried out on conventional male and female white rats (290-320 g, body weight). Each test and control group of animals included eight animals. The strains of microorganisms mentioned above were given to animals by inhalation of minimal effective doses of microbes in the concentrations of 103-108 CFU/m3 during one month. To demonstrate possible adverse effects to gut microflora, the routine bacteriological examination of animal feces was performed. To do this, after the priming, the 10-fold dilutions of animal feces in sterile saline were inoculated onto a set of general-purpose and selective culture media for Enterobacteriaceae members, staphylococci, enterococci, clostridia, bifidobacteria, lactobacilli, and fungi, with subsequent identification of the genus of the isolated microorganism. After that, the concentrations of microorganisms were calculated and measured in lg of CFU/g of feces. The Institutional Ethical Committee of Animal Care and Use of the Pirogov Russian National Research Medical University approved all procedures involving animals. The results of experiments were analyzed with a simple t-test using Statistica (v.6.0, Stat Soft, USA) and Microsoft Office Excel 2007. Results were considered statistically significant when p < 0.05.

Results. The most notable changes in the composition of the intestinal microbiota were observed after inhaling of yeasts of genus Candida at the level of 103–104 CFU/m3 and in cases of exposure to molds (Aspergillus awamori, Penicillium funiculosum, and Tolypocladium cylindrosporum) in the concentration of 2•104 CFU/m3, and gram-negative bacteria of the genus Alcaligenes and genus Pseudomonas at 5•105 CFU/m3. We observed a dramatic decrease of Escherichia coli and the increase of gram-positive bacteria (staphylococci, enterococci). For some genera of biotechnological strains, a significant decline in the content of lactobacilli was also shown. On the other hand, Rhodococcus did not cause any disturbances even at high concentrations in the ambient air.

Conclusion. The obtained data can be used to develop biosafety and hygienic standards for industrial microbes to help decrease or minimize the occupational risk of infection or undesirable allergic effect when working with biotechnological strains of microbes in the ambient air of residential areas.

Hygiene and Sanitation. 2021;100(3):234-239
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Respiratory protective devices for the healthcare workers (literature review)

Kaptsov V.A., Chirkin A.V.

Abstract

Introduction. Healthcare practitioners are at increased risk of infection with infectious diseases, including the inhalation route. Healthcare practitioners use respirators of various designs providing different efficiency of protection.

The purpose of the study was to improve efficiency of the respiratory protection of the healthcare practitioners in Russian Federation.

There were analyzed аvailable NIOSH publications, articles in journals Taylor & Francis, Oxford University Press, published materials of Federal Service for Supervision of Consumer Rights Protection and Human Welfare (Rospotrebnadzor), and western training manuals. Differences in the requirements of the legislation were identified that increase the risk of infection in healthcare practitioners. There are no methods for assessing the risk level, and there are no specific requirements for selecting the respirator’s type that corresponds to the risk level. The employer is not obliged to provide the fit test for all employees. The respirator must be used timely, so it should not negatively affect the worker. But the average carbon dioxide concentration can exceed the STEL by more than two times. The certification requirements for respirators do not correspond to the conditions of their use in the hospitals. Respirators were not certified as means of protection against bioaerosols.

Conclusions. Identified shortcomings in the respiratory safety of health care workers show possible ways to improve their protection by harmonizing national legislation with the best of existing Western requirements.

Hygiene and Sanitation. 2021;100(3):240-245
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Management of sanitary supervision of occupational health at oil refineries and petrochemical plants (analytical review)

Krasovsky V.O., Yakhina M.R.

Abstract

Relevance. Occupational health (factory medicine) as applied medical science provides sanitary inspection practice with theoretical generalizations for rational holding and increase in efficiency of control (supervising) actions. Currently, oil processing and petrochemical plants showed scientific and technical progress with the implementation of new technologies and automation of production, toughening of requirements of industrial safety, and labor protection. But this industry still represents technically difficult huge complexes of the fire and explosion hazardous equipment with harmful intensive chemical and noise effects on the environment and in the first turn, on the workers. Among objects of sanitary inspection in any region of the enterprise, oil processing and petrochemistry Relevance. Occupational health (factory medicine) as applied medical science provides sanitary inspection practice with theoretical generalizations for rational holding and increase in efficiency of control (supervising) actions. Currently, oil processing and petrochemical plants showed scientific and technical progress with the implementation of new technologies and automation of production, toughening of requirements of industrial safety, and labor protection. But this industry still represents technically difficult huge complexes of the fire and explosion hazardous equipment with harmful intensive chemical and noise effects on the environment and in the first turn, on the workers. Among objects of sanitary inspection in any region of the enterprise, oil processing and petrochemistry represent various conglomerates of the main, the auxiliary and providing production of the demanding special receptions in the analysis and preventions of their harm and danger not only to workers, but also to the population.

Aim of the study. To analyze existing hygienic characteristics and requirements to factors of the production environment and work, for the modem enterprises of oil processing and petrochemistry in control (supervising) actions for the increase in efficiency of the leading function of service for managing and supervision in ensuring sanitary and epidemiologic wellbeing of the population of the Russian Federation, consumer protection and rendering the methodical help to practical employees of service.

Conclusions. The most relevant documents are selected and literature sources are listed to optimize the leading function of the Federal Service for Supervision in the Field of Consumer Rights Protection and Human Well-being for control and supervision in the field of ensuring the sanitary and epidemiological well-being of the population of the Russian Federation and consumer rights protection.
various conglomerates of the main, the auxiliary and providing production of the demanding special receptions in the analysis and preventions of their harm and danger not only to workers, but also to the population. Aim of the study. To analyze existing hygienic characteristics and requirements to factors of the production environment and work, for the modem enterprises of oil processing and petrochemistry in control (supervising) actions for the increase in efficiency of the leading function of service for managing and supervision in ensuring sanitary and epidemiologic wellbeing of the population of the Russian Federation, consumer protection and rendering the methodical help to practical employees of service. Conclusions. The most relevant documents are selected and literature sources are listed to optimize the leading function of the Federal Service for Supervision in the Field of Consumer Rights Protection and Human Well-being for control and supervision in the field of ensuring the sanitary and epidemiological well-being of the population of the Russian Federation and consumer rights protection.

Hygiene and Sanitation. 2021;100(3):246-253
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FOOD HYGIENE

Effectiveness of the use of specialized food item among copper industry workers

Dubenko S.E.

Abstract

Introduction. Maintaining health and job longevity and decrease in the Russian working population’s occupational morbidity rate remains a current trend in public policy in health care. The purpose of the research was to evaluate the effectiveness of a new specialized food item with the following definition: “Fruit and vegetable Nectar, enriched with dietary fiber, pectin and vitamins” (from now on “Nectar”) as being healthy nutrition for individuals of auxiliary workshops of the copper industry. 

Materials and methods. Thirty-four workers participated in the research. The questionnaire, clinical and laboratory as well as statistical methods were applied. 

Results. Workers’ diet involved a high proportion of fats (44.6% of the total dietary calories) and insufficient food items sources of ascorbic acid. The share of individuals with an inadequate nutritional supply of vitamins A, C, B1, B2, and PP was 32.6%, 46.9%, 75.5%, 81.6%, and 79.6%, respectively. Low concentration of ascorbic acid in the blood serum (in 32.7% of workers) and normal concentration of vitamin A and B1 in blood were demonstrated in surveyed workers in the winter. The effectiveness of course consumption of Nectar specialized drink was shown in workers. Resulting from its consumption for three or more weeks, statistically significant positive alterations were observed in vitamin status (vitamin A and C), antioxidant defense system (in terms of peroxidase and MDA), together with a subjective health assessment in terms of work capacity and fatigue. Consumption of Nectar reduced cadmium content to environmentally safe level** in workers with high level of cadmium in blood.

Conclusion. Nectar may be used as a therapeutic and prophylactic food item for workers exposed to heavy metals.

 ** Methodical instructions MUC 4.1.1897-04 Atomic absorption measurement of mass concentrations of lead, cadmium, zinc and nickel in the blood (in Russian).

Hygiene and Sanitation. 2021;100(3):254-260
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HYGIENE OF CHILDREN AND ADOLESCENTS

Changes in blood circulation and the central nervous system functions in 4–10 year children with visual impairment

Popova T.V., Kokoreva E.G., Kourova O.G.

Abstract

Introduction. Visual impairment is the most common disturbances during the child development in preschool and primary school age. Such children need care with the health-improving technology based on scientific knowledge about the characteristics of their psychophysiological functions.

The aim of the study is to identify development-related changes in blood circulation and central nervous system functions in 4-10 year healthy children and cases with visual impairments.

Materials and methods. A total of 380 children were examined by electrocardiography, electroencephalography, registration of neurodynamic processes, analysis of the tactile function, and self-assessment of anxiety.

Results. In subjects with visual impairment, an increase in age-related heterochronism in the development of psychophysiological functions was revealed. The accuracy indices of time intervals and tactile sensitivity of the skin of the fingers were higher, and the indices of physical development, kinematometry, and mobility of nervous processes in many age groups are lower than in healthy ones. Such adaptive changes were accompanied by an increase in functional stress. So, for example, in preschool children from 4 to 6 years, when analyzing the structure of the heart rhythm, an increase in the values of the stress index was noted (from 148.08 ± 3.32 to 220.08 ± 3.62 c.u.; р < 0.05).

Conclusion. Judging by the high values ​​of activity indices of the central mechanisms of heart rhythm regulation, anxiety, and the nature of bioelectric changes on the EEG, the “price” of age-related adaptation in children with sensory impairments rises. It contributes to the development of disadaptation. The conclusion is made about the need to develop effective means of psychophysical correction of children with visual impairments, taking into account the nature of age-related changes.

Hygiene and Sanitation. 2021;100(3):261-267
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Dynamics of the functional state of the body of young athletes practicing sports games

Cebanu S.I., Deleu R.D., Tabîrța A.V., Tafuni O.I., Friptuleac G.E.

Abstract

Introduction. Maintaining and strengthening young athletes’ health is very important since a growing body is more sensitive than an adult one. The overload on young athletes’ bodies’ functional systems is much higher compared to their peers who do not engage in intense physical activities and sports. 

This study aimed to assess the functional changes in the body of young athletes practicing sports games in the training process.

Materials and methods. The functional state of the body of young athletes practicing five types of sports (volleyball, basketball, football, tennis, and handball) was studied on a representative group of 94 people, of which 50 (53.2%) males and 44 (46.8%) females with an average age of 17.7 ± 0.19 years. The cardiovascular system, central nervous system, respiratory system, and musculoskeletal system were investigated. The functional state assessment was carried out, taking into account the degree and direction of changes in the dynamics of training of individual indicators and integral indicators. It was determined the degree of risk of developing adverse functional changes. To identify premorbid states, we establish the type of self-regulation of blood circulation.

Results. The obtained results in this study indicate that significant changes express the biological significance of the response of athletes to the conditions of training in the indices of the functions of the cardiovascular and central nervous systems and the musculoskeletal system. The indices of the functional state of the young athletes’ body at the end of the training changed in both directions. The degree of their severity fluctuated within limits from little too clearly expressed values. On the part of the cardiovascular system, an acceleration of heart rate from 60.1 ± 0.93 to 123.1 ± 0.93 beats/min (p < 0.001) was noted, an increase of systolic blood pressure from 101.1 ± 2.84 to 129.2 ± 1.40 mm Hg. (p <0.001), diastolic arterial pressure from 65.8 ± 1.60 to 76.3 ± 1.20 mm Hg. (p < 0.001), pulse pressure from 33.5 ± 2.35 to 71.5 ± 0.85 mm Hg. (p < 0.001), systolic pressure from 78.3 ± 1.75 to 84.2 ± 1.06 ml (p < 0.001), minutes volume from 4.3 ± 0.22 to 10.3 ± 0.11 l (p < 0.001), total peripheral resistance from 934.2 ± 42.11 to 1646.4 ± 37.61 dyn•s/cm5 (p < 0.001). On the part of the central nervous system, there was an increase in visual motor reaction from 236 ± 20.0 to 389 ± 5.3 ms (p < 0.001) and the auditory-motor reaction from 160 ± 3.8 to 256 ± 7.7 ms (p <  0.001). Young athletes who play volleyball have a higher load on the cardiovascular system. Functional changes are more pronounced in female athletes. The risk of developing unfavorable functional changes was noted for 15 physiological indices. The relative risk varied within 1.03-2.45 for boys and 1.04-2.90 for girls. The most pronounced risk is characteristic for total peripheral resistance (RR = 1.60), minutes volume (RR = 1.58), systolic pressure (RR = 1.55) in boys and systolic pressure (RR = 2.35), minutes volume (RR = 2.21), systolic arterial pressure (RR = 1.75), diastolic arterial pressure (RR = 1.73) and peripheral blood pressure (RR = 1.52) in girls.

Conclusions. When playing sports games, a specific professional environment for different types of sports games is formed, this harming the functional state, performance, and health of young athletes.

Hygiene and Sanitation. 2021;100(3):268-273
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Dynamics of morphological and functional characteristics in Yakut youths over 20 years

Ustinova M.V., Stepanova G.K., Nikolaeva I.V., Malysheva L.A.

Abstract

Introduction. Since the end of the last century, changes in society have reflected on the morphophysiological status of the indigenous population of Yakutia. The work investigated the dynamics of the physical constitution and functional indicators of the circulatory system in young Yakuts, students of the North-Eastern Federal University, over the past two decades.

Material and methods. The study involved young men 18-21 years old: 54 born - in 1998-99 and 63 in 2019, anthropometric studies were conducted, and physical development and body type were assessed. Hemodynamic parameters heart rate and blood pressure were determined:

Results. The determination of body mass index (BMI) revealed a high degree of discrepancy between bodyweight and height in students of this century. In 1998-99, the normal BMI value was found in 85.2%, above the norm - in 7.4% of the examined. While in 2019, normal BMI values ​​are observed in 58.7%, in 36.5% - above the norm, including 9.5% - 1-2 degree obesity. In 2019, the distribution of students by somatotype changed: along with a decrease in normosthenics by 14.5% and asthenics by 13.2%, the number of hypersthenics increased by 36.5%. Analysis of hemodynamic parameters depending on the type of constitution revealed significantly higher blood pressure (BP.) values ​​in hypersthenics compared to normostenics and asthenics. Over the 20-year period of observation, students had a significant increase in the values ​​of systolic blood pressure, pulse pressure, and the greatest increase was noted in students with hypersthenic constitution.

Conclusions. The revealed shifts of anthropometric indices in modern Yakut students, compared with the young men of 1998-99, indicate an increase in the prevalence of overweight and the development of a constitution according to the hypersthenic type. Against the background of body hypersthenization, the adaptive capabilities of the circulatory system decrease.

Hygiene and Sanitation. 2021;100(3):274-278
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Risks of developing diseases of an eye and its adnexa in students in conditions of the violation of hygienic rules for the use of electronic devices

Skoblina N.A., Popov V.I., Eryomin A.L., Markelova S.V., Milushkina O.Y., Obrubov S.A., Tsameryan A.P.

Abstract

Introduction. The process of involving children, adolescents and youth in the constantly developing information space is an objective reality. At the same time, the lack of monitored indices of the safety of electronic devices (ED) for the health of children and adolescents, the lack of a clearly built system of preventive measures require its development and improvement, which will reduce the risk of the digital information environment impact on the health of the younger generation.

Materials and methods. The survey using a questionnaire, developed by specialists from the Research Institute of Hygiene and Health Protection of Children and Adolescents in 2017-2020, was used to collect data on the nature of ED using by schoolchildren and students in educational and leisure activities (1218 questionnaires). In 2017-2020, 805 schoolchildren and students from the Moscow gymnasium №12 and the Pirogov Russian National Research Medical University underwent an ophthalmological examination.

Results. All the surveyed schoolchildren and students used ED. Analysis of the questionnaire data allowed us to determine the duration of continuous use of ED (computer, laptop, tablet, smartphone, etc.) by children, adolescents, and young people during school and vacation time. All categories of respondents both during the academic period and during holidays showed a high duration of continuous use of ED. Thus, the presence of a behavioral risk factor for the uncontrolled use of ED (long duration of continuous work with ED) was shown among all categories of respondents, which may cause the development of eye diseases. The analysis of the results obtained revealed significant negative values of the correlation coefficients between students’ visual acuity and the duration of their continuous use of both stationary (computer) and mobile (smartphone, tablet) ED. 

Discussion. The introduction of these preventive measures into the daily routine and lifestyle of children, adolescents, and young people will help prevent visual acuity disorders.

Conclusion. It is necessary to introduce hygienic rationing of the frequency and duration of the possible influence of ED not only during the educational process but also in leisure time to increase the awareness of parents and the degree of their control over the use of ED. To elevate awareness of all ED users’ categories it is necessary to intensify work to promote the rules for the safe use of ED and related foundations of a healthy lifestyle and self-control over ED use.

In connection with the development of the digital educational environment, it is advisable to improve the hygienic standardization of text information in electronic publications for children, adolescents, and students. To prevent the symptoms of eye fatigue, it is advisable to limit continuous work with a bright screen (no more than 60 minutes), followed by a break of at least 15-20 minutes, not related to viewing the illuminated screen, in compliance with the recommendations for relieving stress from the visual analyzer.

Hygiene and Sanitation. 2021;100(3):279-284
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PREVENTIVE TOXICOLOGY AND HYGIENIC STANDARTIZATION

Analysis of toxicity of iron oxide nanocomposite encapsulated in a polymer matrix of arabinogalactan

Titov E.A., Sosedova L.M., Novikov M.A.

Abstract

Introduction. The article presents a toxicity analysis of the Fe3O4 nanocomposite encapsulated in the natural polymer matrix of arabinogalactan (AG). A study was devoted to forming and developing the biological response of organisms to subacute administration of this nanocomposite.

Materials and methods. White outbred male rats weighing 200-220 gr. were used in this study. For ten days, a test drug solution was administered orally to animals with a probe at a dose of 500 μg of iron per kilogram of body weight. Then, using histological and immunohistochemical analysis methods, the severity of the biological response of the organism to the introduction of this nanocomposite was evaluated. An analysis was made of the state of tissue of the liver, kidneys, and sensorimotor cortex. The number of neurons with the proapoptotic caspase three protein expression, anti-apoptotic bcl-2 protein, and HSP 70 stress protein was determined in the sensorimotor cortex tissue.

Results. With intragastric administration to experimental animals, there was a violation of normal blood rheology in liver and kidney tissue, protein dystrophy of hepatocytes, expansion of perivascular spaces of brain tissue, and a decrease in the total number of sensorimotor cortex neurons per unit area. Immunohistochemical analysis of tissue of the sensorimotor cortex for expression of pro-and anti-apoptotic proteins and the expression of HSP 70 protein showed a sharp increase in the number of neurons with the expression of HSP 70 stress protein.

Discussion. The nature of the detected changes indicates the occurrence of compensatory-adaptive reactions in the organism in response to the effect of FeAG. The lack of expression of caspase 3 protein eliminates the development of apoptosis.

Hygiene and Sanitation. 2021;100(3):285-289
pages 285-289 views

Cytokine regulation of inflammatory processes in respiratory organs of rats exposed to the combined inhalation of chemicals in low concentrations

Barantseva M.Y., Mukhamedieva L.N., Dadasheva O.A., Ozerov D.S., Pakhomova A.A., Tsarkov D.S., Lashukov P.V.

Abstract

Introduction. Morphological studies of animals (trachea, bronchi, lungs) exposed to the combined inhalation of chemicals in low concentrations showed the progression of structural changes, indicating the activation of inflammation and fibrosis in the lungs. The role of cytokine markers in developing inflammatory and fibrotic processes and remodeling lung tissue has been studied.

Materials and methods. Male rats (180-200 g) were exposed to a mixture of chemicals (acetone, acetaldehyde, benzene) in low concentrations of 0.7-1.5; 0.9-1.4; 0.2-0.4 (mg/m3), respectively. The concentrations of IL-6, IL-10, IL-1b, IL-4, TGFβ1, TNFα cytokines (pg/ml) have been measured in the lung homogenate by enzyme-linked immunosorbent assay (ELISA). Microscopic anatomy of the lungs, tracheal wall, bronchi has been studied on the 30th day of exposure and the 15th and 90th days of the recovery period. 

Results. An increase in interleukin-4 and transforming growth factor TGFβ1 in the homogenate of the lung tissue was shown. An increase in lymphatic follicles, the number of lymphocytes, neutrophils, macrophages, and focal accumulations of eosinophils has been observed in the tracheal wall. In lymphoid infiltrates of the lung tissue - eosinophils, macrophages, and plasmocytes. Accumulation of eosinophilic exudate has been observed in some alveoli. The 90th day of the recovery period is characterized by a significant increase of TGFβ1 in the lung tissue, indicating fibrosis, as evidenced by the rise in the number of fibroblasts between the alveoli in the atelectasis zones of lungs.

Conclusion. The chronic combined exposure to the mixture of chemicals in low concentrations is accompanied by a pro-inflammatory process in the lungs with the type II hypersensitivity and increasing IL-4 and TGFβ1 (a key mediator of profibrotic activity).

Hygiene and Sanitation. 2021;100(3):290-294
pages 290-294 views