Pyrogenic compounds in house dust of Moscow dwellings

Exposure to pyrogenic compounds are essential characteristics of dwellings. This is important for people with sensitization, especially to airborne allergens. The aim of this work was to study the exposure of LPS and beta-glucans in house dust of Moscow dwellings. The determination of the LPS concentration was implemented with the use of LAL-test. The content of beta-glucans was determined with the use both of the “Glucatell”-test and the new inhibitor immunoassay method, which allows identify generally linear beta-glucans and their fragments. The method is based on the use of polyclonal rabbit antiserum obtained against the conjugate nona-β-(1→3)-D-glucoside with bovine serum albumin, and the inhibitory agent is nona-β-(1→3)-D-glucoside. The LPS concentration varied from 0.6 to 6 µg/g dust, mean concentration was equal to 2.19±1.74 µg/g dust (M=1.2 µg/g dust). The concentration of beta-glucans in “Glucatell”-test ranged from 8 to 116 µg /g dust, mean concentration was 75.58±25.27 µg/g dust (M=77 µg/g of dust), the concentration of beta-glucans in ILISA ranged from 10 to 109 µg/g dust, mean content was of 28.45±14.12 µg/g dust (M=24 µg/g dust). LPS exposure did not exceed 1.2 µg/g of dust in 65% of the apartments. Such concentrations of LPS in the environment of sensitized people can stimulate the development of allergic reactions. There was a trend in the detection of higher exposure of beta-glucans by using “Glucatell” test, which ranged from 40 to 100 µg/g of dust in 76% of the apartments when using the ILISA in 84% of the apartments in the content of beta-glucans ranged from 11 to 40 µg/g of dust. The correlation between data obtained by different methods was absent (r=0.2056, p<0.05). Thus, the different methods allow determine pyrogenic compounds of different structure. Therefore, these indices are independent of the characteristics of pyrogenic environment. The determination of the concentration of each of them in the same room seems to be reasonable.