Transcriptional activity of metallothionein genes in acute poisoning caused by cadmium chloride

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Introduction. Cadmium is one of the most toxic heavy metals widely distributed in the environment. It has a long half-life, leading to its accumulation in various organs, which in turn is the cause of many adverse effects on human health.

Material and methods. Acute toxic poisoning with cadmium chloride was simulated on white outbred male rats, divided into groups depending on the exposure time. Samples of liver and kidney tissues were used as study materials, in the homogenate of which the level of mRNA of metallothionein genes was determined.

Results. It was found that the maximum value of the multiplicity of MT1 gene expression in the liver was reached after 6 hours (16.36 ± 0.77; p <0.001), and in the kidneys one day after intoxication with cadmium chloride (6.12 ± 0.43; p <0.001). The activity of the MT2 gene in the liver was most pronounced in the range of 2-4 hours (14.35 ± 1.73; 14.78 ± 1.44; p <0.001), while in the renal tissues an increase in the amount of mRNA was recorded after 24 hours (7.32 ± 0.63; p <0.001). The level of MT3 gene transcripts in liver tissues was decreased throughout the experiment, however, the opposite picture was observed in the kidney tissue, where the maximum was reached one day after the administration of the toxicant (6.14 ± 0.31; p <0.001).

Conclusion. Thus, an increase in metallothionein expression in response to the presence of heavy metal ions in the body can be used as a genetic marker in case of poisoning with various compounds.

Sobre autores

Munira Ziatdinova

Ufa Research Institute of Occupational Health and Human Ecology

Autor responsável pela correspondência
Email: munira.munirovna@yandex.ru
ORCID ID: 0000-0002-1848-7959

MD, junior researcher of the Department of Toxicology and Genetics with an experimental laboratory of laboratory animals, Ufa Research Institute of Occupational Health and Human Ecology, Ufa, 450106, Russian Federation.

у-mail: munira.munirovna@yandex.ru

Rússia

Yana Valova

Ufa Research Institute of Occupational Health and Human Ecology

Email: noemail@neicon.ru
ORCID ID: 0000-0001-6605-9994
Rússia

Guzel Mukhammadiyeva

Ufa Research Institute of Occupational Health and Human Ecology

Email: noemail@neicon.ru
ORCID ID: 0000-0002-7456-4787
Rússia

Elza Usmanova

Ufa Research Institute of Occupational Health and Human Ecology

Email: noemail@neicon.ru
ORCID ID: 0000-0002-5455-6472
Rússia

Denis Karimov

Ufa Research Institute of Occupational Health and Human Ecology

Email: noemail@neicon.ru
ORCID ID: 0000-0003-0039-6757
Rússia

Nadezhda Khusnutdinova

Ufa Research Institute of Occupational Health and Human Ecology

Email: noemail@neicon.ru
ORCID ID: 0000-0001-5596-8180
Rússia

Samat Bajgil’Din

Ufa Research Institute of Occupational Health and Human Ecology

Email: noemail@neicon.ru
ORCID ID: 0000-0002-1856-3173
Rússia

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Declaração de direitos autorais © Ziatdinova M.M., Valova Y.V., Mukhammadiyeva G.F., Usmanova E.N., Karimov D.O., Khusnutdinova N.Y., Bajgil’Din S.S., 2024


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