Organ-specific expression of protective proteins under the conditions of dust exposure to the body (experimental study)

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Abstract

The objective of the study was to evaluate the organ-specific features of the expression of protective proteins of the HSP family in response to prolonged dust exposure in an experiment.

Material and methods. The experiments were performed on 60 male Wistar rats weighing 200-250 g. The animals were divided into groups: rats inhaled coal-rock dust in the priming dust chamber for 4 hours daily for 6 weeks (average concentration 50 mg/m3), and the control rats (they were in an equal volume chamber, where the same temperature and air exchange conditions were maintained, but without the supply of coal-rock dust). In the cytoplasmic fraction of the lungs, heart, liver, and brain, the levels of HSP72 and heme oxygenase-1 (HOx-1) were determined by Western blot analysis.

Results. The prolonged exposure of coal-rock dust to the body changed the level of intracellular proteins HSP72 and HOx-1 in the lungs, heart, liver, and brain. An increase in both HSP72 and HOx-1 levels occurred in the lungs and brain tissue; a significant increase in HOx-1 was recorded in the heart and HSP72 in the liver. These data indicate the organ-specific expression of intracellular proteins during dust exposure to the body. The following molecular mechanisms are involved in the development of the response to prolonged inhalation of coal-rock dust: 1) in the lungs and brain - both stress (HSP72) and hypoxic (HOx-1) components; 2) in the heart - a hypoxic component, manifested by the intensive synthesis of HOx-1 throughout the study period; 3) in the liver - a stress component due to significant expression of HSP72, which is associated with the manifestation of a protective effect for both the organ itself and the body as a whole.

Conclusion. The results obtained indicate the organ-specificity of the cellular response of the body to the prolonged exposure to industrial dust. A change in the expression level of HSP72 and HOx-1 characterizes the degree of protection of organs from damage caused by inhalation of coal-rock dust, which grows in the series as liver < heart < lungs and brain.

About the authors

Anna G. Zhukova

Research Institute for Complex Problems of Hygiene and Occupational Diseases; Novokuznetsk Institute (Branch Campus) of the Kemerovo State University

Author for correspondence.
Email: nyura_g@mail.ru
ORCID iD: 0000-0002-4797-7842
MD, Ph.D., DSci., head of the laboratory for molecular-genetic and experimental researches, Research Institute for Complex Problems of Hygiene and Occupational Diseases, Novokuznetsk, 654041, Russian Federation. Russian Federation

Natalya N. Zhdanova

Research Institute for Complex Problems of Hygiene and Occupational Diseases

Email: noemail@neicon.ru
Russian Federation

Anastasiуa S. Kazitskaya

Research Institute for Complex Problems of Hygiene and Occupational Diseases

Email: noemail@neicon.ru
ORCID iD: 0000-0001-8292-4810
Russian Federation

Nadezhda N. Mikhailova

Research Institute for Complex Problems of Hygiene and Occupational Diseases; Novokuznetsk Institute (Branch Campus) of the Kemerovo State University

Email: noemail@neicon.ru
ORCID iD: 0000-0002-1127-6980
Russian Federation

Tat'yana G. Sazontova

Faculty of Fundamental Medicine of Lomonosov Moscow State University

Email: noemail@neicon.ru
Russian Federation

References

  1. Chebotarev A.G. Working environment and occupational morbidity of mine personnel. Gornaya promyshlennost’. 2018; (1): 92–5. http://doi.org/10.30686/1609-9192-2018-1-137-92-95 (in Russian)
  2. Oleshchenko A.M., Zakharenkov V.V., Surzhikov D.V., Panaiotti E.A., Tsay L.V. Evaluating risk of morbidity among workers of coal open-cast mines in Kuzbass. Meditsina truda i promyshlennaya ekologiya. 2006; (6): 13–6. (in Russian)
  3. Voroshilov Ya.S., Fomin A.I. Impact of coal dust on the professional morbidity of coal industry workers. Ugol’. 2019; (4): 20–4. https://doi.org/10.18796/0041-5790-2019-4-20-2 (in Russian)
  4. Perret J.L., Plush B., Lachapelle P., Hinks T.S., Walter C., Clarke P. et al. Coal mine dust lung disease in the modern era. Respirology. 2017; 22(4): 662–70. https://doi.org/10.1111/resp.13034
  5. Velichkovskiy B.T. Pathogenic classification of occupational respiratory diseases caused by exposure of fibrogenic dust. Pul’monologiya. 2008; (4): 93–101. (in Russian)
  6. Fomenko D.V., Ulanova E.V., Gromov K.G., Kazitskaya A.S., Bondarev O.I. Medical and biologic research of coal dust exposure as intoxication factor. Byulleten’ VSNTs SO RAMN. 2006; (1): 278–83. (in Russian)
  7. Shan H., Li T., Zhang L., Yang R., Li Yu., Zhang M., et al. Heme oxygenase-1 prevents heart against myocardial infarction by attenuating ischemic injury-induced cardiomyocytes senescence. EBioMedicine. 2019; 39: 59–68. https://doi.org/10.1016/ j.ebiom.2018.11.056
  8. Pinho R.A., Bonatto F., Andrades M., Frota M.L., Ritter C., Klamt F. et al. Lung oxidative response after acute coal dust exposure. Environ Res. 2004; 96(3): 290–7. https://doi.org/10.1016/j.envres.2003.10.006
  9. Xing J.C., Chen W.H., Han W.H., Guo M.F., Rehn S., Bruch J. Changes of tumor necrosis factor, surfactant protein A, and phospholipids in bronchoalveolar lavage fluid in the development and progression of coal workers’ pneumoconiosis. Biomed Environ Sci. 2006; 19(2): 124–9.
  10. Ulker O., Yucesoy B., Demir O., Tekin I., Karakaya A. Serum and BAL cytokine and antioxidant enzyme levels at different stages of pneumoconiosis in coal workers. Hum Exp Toxicol. 2008; 27(12): 871–7. https://doi.org/10.1177/0960327108098332
  11. Pandey J.K., Agarwal D. Biomarkers: A potential prognostic tool for silicosis. Indian J Occup Environ Med. 2012; 16(3): 101–7. https://doi.org/10.4103/0019-5278.111746
  12. Zhukova A.G., Mikhaylova N.N., Sazontova T.G., Zhdanova N.N., Kazitskaya A.S., Bugaeva M.S. et al. Participation of free-radical processes in structural and metabolic disordes of lung tissues in the dynamics of coal-rock dust exposure and the adaptogenic correction. Byulleten’ eksperimental’noy biologii i meditsiny. 2019; 168(10): 420–24. (in Russian)
  13. Zakharenkov V.V., Mikhaylova N.N., Zhdanova N.N., Gorokhova L.G., Zhukova A.G. Experimental study of the mechanisms of intracellular defense in cardiomyocytes associated with stages of anthracosilicosis development. Bulletin of Byulleten’ eksperimental’noy biologii i meditsiny. 2019; 159(4): 418–22. (in Russian)
  14. Xing J.C., Chen W.H., Wang F., Han W.H., Ren H.M., Wu T.C. [Relationship between dust exposure and chronic obstructive pulmonary diseases and heat shock protein 72 and 73 in lymphocytes among coal miners]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2006; 24(9): 540–3. (in Chinese)
  15. Wang H., Xing J., Wang F., Han W., Ren H., Wu T. et al. Expression of Hsp27 and Hsp70 in lymphocytes and plasma in healthy workers and coal miners with lung cancer. J Huazhong Univ Sci Technol Med Sci. 2010; 30(4): 415–20. https://doi.org/10.1007/s11596-010-0441-5
  16. Gorokhova L.G., Bugaeva M.S., Ulanova E.V., Fomenko D.V., Kizichenko N.V., Mikhaylova N.N. Method of priming laboratory animals with industrial dust for modeling silicosis. Patent RF № 2546096; 2015. (in Russian)
  17. Arkhipenko Yu.V., Didenko V.V., Sazontova T.G., Meerson F.Z. Comparative assessment of the effect of immobilization stress on the dynamics of resistance to induction of lipid peroxidation of internal organs and the brain. Doklady Akademii nauk SSSR. 1989; 304(6): 1500–3. (in Russian)
  18. Mikhaylova N.N., Zakharenkov V.V., Bugaeva M.S., Bondarev O.I., Kazitskaya A.S., Kizichenko N.V. et al. Specificity of morphologic changes in target organs associated with exposure to coal rock dust and fluorine compounds. Meditsina truda i promyshlennaya ekologiya. 2016; (5): 11–5. (in Russian)
  19. Belkina L.M., Lakomkin V.L., Zhukova A.G., Kirillina T.N., Saltykova V.A., Sazontova T.G. et al. Heart resistance to oxidative stress in rats of different genetic strains. Byulleten’ eksperimental’noy biologii i meditsiny. 2004; 138(3): 219–22. https://doi.org/10.1007/s10517-005-0003-1
  20. Andreeva L.I., Boykova A.A., Margulis B.A. Peculiarities of intracellular content and functional role of heat shock proteins 70 kDa under stress and adaptation. Tekhnologii zhivykh sistem. 2009; 6(3): 11–8. (in Russian)
  21. Mikhaylova N.N., Sazontova T.G., Alekhina D.A., Kazitskaya A.S., Zhdanova N.N., Prokop’ev Yu.A. et al. Features of intracellular protective mechanisms under the action of various xenobiotics on the body. Tsitokiny i vospalenie. 2013; 12(4): 71–5. (in Russian)
  22. Zhukova A.G., Kazitskaya A.S., Sazontova T.G., Mikhaylova N.N. Hypoxia-inducible factor (HIF): structure, function, and genetic polymorphism. Gigiena i sanitariya. 2019; 98(7): 723–8. https://doi.org/10.18821/0016-9900-2019-98-7-723-728 (in Russian)
  23. Pittala V., Vanella L., Salerno L., Romeo G., Marrazzo A., Di Giacomo C. et al. Effects of polyphenolic derivatives on heme oxygenase-system in metabolic dysfunctions. Curr Med Chem. 2018; 25(13): 1577–95. https://doi.org/10.2174/0929867324666170616110748
  24. Vona R., Gambardella L., Cittadini C., Straface E., Pietraforte D. Biomarkers of oxidative stress in metabolic syndrome and associated diseases. Oxid Med Cell Longev. 2019; 2019: 8267234. https://doi.org/10.1155/2019/8267234
  25. Evdonin A.L., Medvedeva N.V. The extracellular heat shock protein 70 and its functions. Tsitologiya. 2009; 51(2): 130–7. (in Russian)
  26. Cornelussen R.N., Knowlton A.A. Heme-oxygenase-1: versatile sentinel against injury. J Mol Cell Cardiol. 2002; 34(10): 1297–300. https://doi.org/10.1006/jmcc.2002.2099
  27. Melling C.W., Thorp D.B., Milne K.J., Krause M.P., Noble E.G. Exercise-mediated regulation of Hsp70 expression following aerobic exercise training. Am J Physiol Heart Circ Physiol. 2007; 293(6): H3692–8. https://doi.org/10.1152/ajpheart.00827.2007
  28. Tsan M.F., Gao B. Heat-shock proteins and immune system. J Leukoc Biol. 2009; 85(6): 905–10. https://doi.org/10.1189/jlb.0109005
  29. Garbuz D.G., Evgen’ev M.B. The evolution of heat shock genes and expression patterns of heat shock proteins in the species from temperature contrasting habitats. Genetika. 2017; 53(1): 12–30. https://doi.org/10.7868/S0016675817010064 (in Russian)
  30. Hfaiedh N., Allagui M.S., El Feki A., Gaubin Y., Murat J.C., Soleilhavoup J.P. et al. Effects of nickel poisoning on expression pattern of the 72/73 and 94 kDa stress proteins in rat organs and in the COS-7, HepG2, and A549 cell lines. J Biochem Mol Toxicol. 2005; 19(1): 12–8. https://doi.org/10.1002/jbt.20056

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Copyright (c) 2024 Zhukova A.G., Zhdanova N.N., Kazitskaya A.S., Mikhailova N.N., Sazontova T.G.


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