Study of features of bioaccumulation and pathomorphological changes in tissues of rat organs after a single inhalation exposure to molybdenum (VI) oxide nanoparticles in comparison with a microdisperse analogue

Cover Page

Cite item

Full Text

Abstract

Introduction. Nanoparticles of molybdenum (VI) oxide (MoO3 NPs) are used in the production of nanooptics, products of the electrochemical, textile and chemical industries. Currently, the possibility of their application in the fields of oil refining and nanoelectronics is also being considered. Expanding the range of application of MoO3 NPs can lead to air pollution, exposure of the population and the development of negative effects due to the toxic properties of this nanomaterial. In this regard, there is a need to study the toxicity of MoO3 NPs under the inhalation.

Materials and methods. The size, surface area, and total pore volume of MoO3 NPs were determined. A study included assessing of bioaccumulation and pathomorphological changes in tissues of rats organs after a single inhalation exposure to MoO3 NPs compared with a microsized chemical analogue.

Results. The NPs size was 662.5 nm, which is 5.15 times less than microparticles (MP) (3410 nm). The surface area of the nanomaterial is 3.66 m2/g, which is 1.17 times more than MPs (3.14 m2/g); the total volume of pores located on the surface of NPs was 0.0133 cm3/g, which exceeds this parameter in NPs by 1.18 times (0.0113 cm3/g). An increase in the concentration of molybdenum in the heart, lungs, liver, kidneys and brain of rats 14 days after single inhalation exposure to MoO3 NPs and MPs was not found. Pathological changes in the tissues of lungs, brain and liver of exposed rats were established. A more pronounced toxic effect of NPs in comparison with MPs was shown in acute plethora and the development of reactive follicles in the lungs.

Limitations. The study was performed only with a single inhalation administration of NPs and MPs of MoO3 in Wistar rats. 

Conclusion. The tested material is a product of the nanoindustry. It does not possess of bioaccumulation after single inhalation exposure. It causes more pronounced pathomorphological changes in the lung tissues in comparison with the micromaterial. The obtained results should be taken into account when developing preventive measures for workers and the public exposed to MoO3 NPs and improving the methodology for hygienic regulation of new chemicals.

Compliance with ethical standards. The study was performed in accordance with the European Convention for the Protection of Vertebrate Animals used for Experimental or other Scientific Purposes (ETS No. 123) and the requirements of the Ethics Committee of the Federal Scientific Center for Medical and Preventive Health Risk Management Technologies (Protocol No. 1, dated January 15, 2021).

Contribution:
Zaitseva N.V. — the concept and design of the study, statistical processing of the material, editing;
Zemlyanova M.A. — the concept and design of the study, processing of the material, writing the text;
Stepankov M.S. — collection of material, writing the text;
Ignatova A.M., Nikolaeva A.E. — processing of the material.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.

Conflict of interest. The authors declare no conflict of interest.

Acknowledgement. The study had no sponsorship.

Received: April 11, 2022 / Accepted: June 8, 2022 / Published: June 26, 2022

About the authors

Nina V. Zaitseva

Federal Scientific Center for Medical and Preventive Health Risk Management Technologies

Author for correspondence.
Email: noemail@neicon.ru
ORCID iD: 0000-0003-2356-1145
Russian Federation

Marina A. Zemlyanova

Federal Scientific Center for Medical and Preventive Health Risk Management Technologies; Perm State National Research University; Perm National Research Polytechnic University

Email: zem@fcrisk.ru
ORCID iD: 0000-0002-8013-9613

MD, PhD, DSci., Professor, Head of Biochemical and Cytogenetic Diagnostic Techniques Department, Federal Scientific Center for Medical and Preventive Health Risk Management Technologies, Perm, 614045, Russian Federation.

e-mail: zem@fcrisk.ru

Russian Federation

Mark S. Stepankov

Federal Scientific Center for Medical and Preventive Health Risk Management Technologies

Email: noemail@neicon.ru
ORCID iD: 0000-0002-7226-7682
Russian Federation

Anna M. Ignatova

Federal Scientific Center for Medical and Preventive Health Risk Management Technologies; Institute of Continuum Mechanics of the Ural Branch of the Russian Academy of Sciences

Email: noemail@neicon.ru
ORCID iD: 0000-0001-9075-3257
Russian Federation

Alena E. Nikolaeva

Federal Scientific Center for Medical and Preventive Health Risk Management Technologies

Email: noemail@neicon.ru
ORCID iD: 0000-0003-3119-3477
Russian Federation

References

  1. Bandman A.L., Volkova N.V., Grekhova T.D., Gudzovskiy G.A., Davydova V.I., Dvorkin E.L., et al. Harmful Chemicals. Inorganic Compounds of Groups V–VIII: Reference Edition [Vrednye khimicheskie veshchestva. Neorganicheskie soedineniya V–VIII grupp: Spravochnoe izdanie]. Leningrad: Khimiya; 1989. (in Russian)
  2. Stone V., Miller M.R., Clift M.J.D., Elder A., Mills N.L., Møller P., et al. Nanomaterials versus ambient ultrafine particles: an opportunity to exchange toxicology knowledge. Environ. Health Perspect. 2017; 125(10): 106002. https://doi.org/10.1289/EHP424
  3. Smita S., Gupta S.K., Bartonova A., Dusinska M., Gutleb A.C., Rahman Q. Nanoparticles in the environment: assessment using the casual diagram approach. Environ. Health. 2012; 11(Suppl. 1): 1–11. https://doi.org/10.1186/1476-069X-11-S1-S13
  4. Rönkkö T., Timonen H. Overview of sources and characteristics of nanoparticles in urban traffic-influenced areas. J. Alzheimer’s Dis. 2019; 72(1): 15–28. https://doi.org/10.3233/JAD-190170
  5. Sukhanova A., Bozrova S., Sokolov P., Berestovoy M., Karaullov A., Nabiev I. Dependence of nanoparticle toxicity on their physical and chemical properties. Nanoscale Res. Lett. 2018; 13(44): 1–21. https://doi.org/10.1186/s11671-018-2457-x
  6. Khan Ib., Saeed K., Khan Id. Nanoparticles: properties, applications and toxicities. Arabian J. Chem. 2019; 12(7): 908–31. https://doi.org/10.1016/j.arabjc.2017.05.011
  7. Parenago O.P., Bakunin V.N., Kuz’mina G.N., Suslov A.Yu., Vedeneeva L.M. Molybdenum sulfide nanoparticles as new-type additives to hydrocarbon lubricants. Doklady akademii nauk. 2002; 383(1): 86–6.
  8. Sobańska Z., Zapór L., Szparaga M., Stȩpnik M. Biological effects of molybdenum compounds in nanosized forms under in vitro and in vivo conditions. Int. J. Occup. Med. Environ. Health. 2020; 33(1): 1–19. https://doi.org/10.13075/ijomeh.1896.01411
  9. Lee S.H., Kim Y.H., Deshpande R., Parilla P.A., Whitney E., Gillaspie D., et al. Reversible lithium-ion insertion in molybdenum oxide nanoparticles. Adv. Mater. 2008; 20(19): 3627–32. https://doi.org/10.1002/adma.200800999
  10. Indrakumar J., Korrapati P.S. Steering efficacy of nano molybdenum towards cancer: Mechanism of action. Biolog. Trace Element Res. 2020; 194(1): 121–34. https://doi.org/10.1007/s12011-019-01742-2
  11. Božinović K., Nestić D., Centra U.G., Ambriović-Ristov A., Dekanić A., de Bisschop L., et al. In-vitro toxicity of molybdenum trioxide nanoparticles on human keratinocytes. Toxicology. 2020; 444: 15264. https://doi.org/10.1016/j.tox.2020.152564
  12. Tran T.A., Krishnamoorthy K., Song Y.W., Cho S.K., Kim S.J. Toxicity of nano molybdenum trioxide toward invasive breast cancer cells. ACS Appl. Mater. Interfaces. 2014; 6(4): 2980–6. https://doi.org/10.1021/am405586d
  13. Assadi F., Amirmoghadami H.R., Shamseddin M., Nedaei K., Heidari A. Effect of molybdenum trioxide nanoparticles (MoO3 NPs) on thyroid hormones in female rats. J. Human Environ. Health Prom. 2016; 1(4): 189–95. https://doi.org/10.29252/jhehp.1.4.189
  14. Sizova E.A., Miroshnikov S.A., Kalashnikov V.V. Morphological and biochemical parameters in wistar rats influenced by molybdenum and its oxide nanoparticles. Sel’skokhozyaystvennaya biologiya. 2016; 51(6): 929–36. https://doi.org/10.15389/agrobiology.2016.6.929rus (in Russian)
  15. Fazelipour S., Assadi F., Tootian Z., Sheibani M.T., Dahmardeh M., Zentabvar O., et al. Effect of molybdenum trioxide nanoparticles on histological changes of uterus and biochemical parameters of blood serum in rat. Comparative Clin. Pathol. 2020; 29(6): 991–9. https://doi.org/10.1007/s00580-020-03137-5
  16. Gmoshinskiy I.V., Bagryantseva O.V., Khotimchenko S.A. Toxicological and hygienic assessment of titanium dioxide nanoparticles as a component of e171 food additive (review of the literature and metahanalysis). Analiz riska zdorov’yu. 2019; (2): 145–63. https://doi.org/10.21668/health.risk/2019.2.17 (in Russian)
  17. Gmoshinskiy I.V., Bagryantseva O.V., Arnautov O.V., Khotimchenko S.A. Nanoclays in food products: benefits and possible risks (literature review). Analiz riska zdorov’yu. 2020; (1): 142–64. https://doi.org/10.21668/health.risk/2020.1.16 (in Russian)
  18. Gmoshinskiy I.V., Khotimchenko S.A. Assessing risks caused by nickel-containing nanomaterials: hazard characterization in vivo. Analiz riska zdorov’yu. 2021; (3): 177–91. https://doi.org/10.21668/health.risk/2021.3.18 (in Russian)
  19. Gregg S.J., Sing K.S.W. Adsorption, Surface Area and Porosity. Academic press; 1982.
  20. Barrett E.P., Joyner L.G., Halenda P.P. The determination of pore volume and area distributions in porous substances. I. Computations from nitrogen isotherms. J. Am. Chem. Soc. 1951; 73: 373–80.
  21. Kapp Jr. R.W. Molybdenum. In: Encyclopedia of toxicology (Third edition). Elsevier Inc.; 2014: 383–8.
  22. Lebedev S., Yasheva E., Galaktionova L., Sizova E. Impact of molybdenum nanoparticles on survival, activity of enzymes, and chemical elements in Eisenia fetida using test on artificial substrata. Environ. Sci. Pollution Res. Internat. 2016; 23(18): 18099–110. https://doi.org/10.1007/s11356-016-6916-6
  23. Pham-Huy L.A., He H., Pham-Huy C. Free radicals, antioxidants in disease and health. Internat. J. Biomed. Sci. 2008; 4(2): 89–96.
  24. Phanindra A., Jestadi D.B., Periyasamy L. Free radicals: properties, sources, targets, and their implication in various diseases. Indian J. Clin. Biochem. 2015; 30(1): 11–26. https://doi.org/10.1007%2Fs12291-014-0446-0
  25. Sirajuddin A., Raparia K., Lewis V.A., Franks T.J., Dhand S., Galvin J.R., et al. Primary pulmonary lymphoid lesions: radiologic and pathologic findings. Radiographics. 2016; 36(1): 53–70. https://doi.org/10.1148/rg.2016140339
  26. Kaptein F.H.J., Kroft L.J.M., Hammerschlag G., Ninaber M.K., Bauer M.P., Huisman M.V., et al. Pulmonary infarction in acute pulmonary embolism. Thromb. Res. 2021; 202: 162–9. https://doi.org/10.1016/j.thromres.2021.03.022
  27. Overview of vasculitis. MSD Manual professional version. Available at: https://www.msdmanuals.com/professional/musculoskeletal-and-connective-tissue-disorders/vasculitis/overview-of-vasculitis
  28. Fuller G., Manford M. Subarachnoid hemorrhage. In: Neurology (Third edition). Elsevier Inc; 2010: 72–3.
  29. Strukov A.I., Serov V.V. Pathological Anatomy: Textbook [Patologicheskaya anatomiya: uchebnik]. Moscow: Litterra; 2010. (in Russian)
  30. Malova I.Yu. The General Doctrine of Dystrophies (Methodical Manual) [Obshchee uchenie o distrofiyakh (metodicheskoe posobie)]. Maykop; 2014. (in Russian)
  31. Tsyrkunov V.M., Prokopchik N.I., Andreev V.P., Kravchuk R.I. Clinical morphology of liver: dystrophies. Gepatologiya i gastroenterologiya. 2017; 1(2): 140–51. (in Russian)
  32. Braydich-Stolle L., Hussain S., Schlager J.J., Hoffman M.C. In vitro cytotoxicity of nanoparticles in mammalian germline stem cells. Toxicol. Sci. 2005; 88(2): 412–9. https://doi.org/10.1093/toxsci/kfi256
  33. Abdelhalim M.A.K., Jarrar B.M. Gold nanoparticles induced cloudy swelling to hydropic degeneration, cytoplasmic hyaline vacuolation, polymorphism, binucleation, karyopyknosis, karyolysis, karyorrhexis and necrosis in the liver. Lipids Health Dis. 2011; 10: 166. https://doi.org/10.1186/1476-511X-10-166

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2024 Zaitseva N.V., Zemlyanova M.A., Stepankov M.S., Ignatova A.M., Nikolaeva A.E.


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 37884 от 02.10.2009.