Assessment of changes in the main homeostasis indicators in mammals after hyperthermic exposure

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Abstract

Introduction. The occupation of firefighters is characterized by the impact of both hazardous fire factors and a complex of harmful and dangerous characteristics of various natures, often exceeding the expected values. High air temperature is typical for almost all types of fire and is one of the main adverse physical factors affecting the body of firefighters. Experimental models on animals are of leading importance in studying extreme fire factors, including hyperthermic exposure.

Material and methods. The study was carried out on 124 outbred white male rats of 3 months of age weighing 250-300 g, divided randomly into two groups. The first group (62 rats) was subjected to a single hyperthermic load. The second group (62 rats) was subjected to daily hyperthermic load during 14 days. 

The study of the leading indicators of the acid-base state and water-electrolyte balance, biochemical and clinical blood tests, cellular and humoral immunity was carried out before and after hyperthermic exposure.

Results. The obtained data indicate high ambient temperatures cause changes in homeostasis indices in laboratory animals. At the same time, there are alterations in the indicators of water-electrolyte balance, acid-base state, clinical and biochemical blood tests and the immune system. 

Conclusion. An experimental model of extreme heat exposure on animals showed the emergence and persistence of changes in the leading indicators of homeostasis. In the group of single hyperthermia, these parameters were restored to the background levels in a day. In the group of multiple hyperthermia, they remained altered.

Contribution:

Kriyt V.E. — the concept and design of the study, collection and processing of material, statistical processing, writing a text;

Sladkova Yu.N. — collection and processing of material, writing a text;

Sannikov M.V. — editing;

Pyatibrat A.O. — collection and processing of material, statistical processing.

All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.

Conflict of interest. The authors declare no conflict of interest.

Acknowledgement. The study had no sponsorship.

The conclusion of the committee on biomedical ethics: the Local ethics committee of the North-West Public Health Research Center of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing approved this study carried out under the European Convention for the Protection of Vertebrate Animals Used for Experiments or Other Scientific Purposes (ETS N 123), Directive of the European Parliament and Council of the European Union 2010/63/EU of 22.09.2010 on the protection of animals used for scientific purposes.

Received: October 19, 2021 / Accepted: November 25, 2021 / Published: December 30, 2021

About the authors

Vladimir E. Kriyt

North-West Public Health Research Center

Author for correspondence.
Email: v.kriit@s-znc.ru
ORCID iD: 0000-0002-1530-4598

MD, PhD, head of the Department of Complex Hygienic Assessment of Physical Factors. North-West Public Health Research Center, Saint-Petersburg, 191036, Russian Federation.

e-mail: v.kriit@s-znc.ru

Russian Federation

Yuliya N. Sladkova

North-West Public Health Research Center

Email: noemail@neicon.ru
ORCID iD: 0000-0003-1745-2663
Russian Federation

Maksim V. Sannikov

Nikiforov Russian Center for Emergency and Radiation Medicine

Email: noemail@neicon.ru
ORCID iD: 0000-0003-3969-9501
Russian Federation

Aleksandr O. Pyatibrat

St. Petersburg State Pediatric Medical University

Email: noemail@neicon.ru
ORCID iD: 0000-0001-6285-1132
Russian Federation

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