Effect of chronic stress on the degree of acrylamide toxicity in rats

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Abstract

Introduction. Chronic stress plays an important role in the development of a wide range of mental, somatic and behavioural disorders, and therefore is a risk factor for the health in living organisms. A negative effect on the state of the nervous system is also observed when the body is poisoned with acrylamide, which is a substance of the second class of danger and is considered toxic and carcinogenic. Exposure of the body to mental and toxic stressors leads to autonomic and neuroendocrine activation, which, in turn, manifests itself as special behavioural patterns.

The purpose of the experiment was to assess the effect of chronic stress on the degree of acrylamide toxicity in rats.

Materials and methods. The experiment was conducted on white outbred rats with an average weight of 200 g of both sexes (n=60), which were evenly distributed into five groups: negative control, chronic stress, acrylamide, acrylamide + treatment, acrylamide + chronic stress. Throughout the study, the animals were kept under standard conditions with 12 hours of artificial lighting during the day, a relatively constant level of humidity (30–70%) and an air temperature of 20–25 °C. Behavioural tests were conducted once a week for one calendar month using a 40×40 hole board, a multifunctional cage to assess general activity, and an elevated plus maze with an ANY-maze video tracking system. Biochemical parameters were assessed in accordance with the manufacturer’s instructions.

Results. Analysis of behaviour on a board with holes did not reveal statistically significant results (H=8.987; p=0.061). When comparing vertical and horizontal motor activity between groups, statistically significant differences were found (p<0.05). AST levels were higher in the stress groups, while cholesterol levels in the same groups were lower (p<0.05).

Limitations. Laboratory animals of only one biological species were used for the experiment. The toxicant was used only in one concentration.

Conclusion. Chronic stress may influence on acrylamide toxicity in rats to a certain extent.

Compliance with ethical standards. Date of the meeting of the bioethical commission of the Federal Budgetary Institution “Ufa Research Institute of Occupational Medicine and Human Ecology” 08.02.2024, No. 01-02. Manipulations with all animals were carried out strictly in compliance with the rules prescribed in basic regulatory documents, including the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes (Strasbourg, 1986) and the Declaration of Helsinki on the Humane Treatment of Animals.

Contribution:
Gizatullina A.A. — concept and design of the study, collection and processing of material, statistical processing, text writing;
Khusnutdinova N.Yu. — collection and processing of material;
Karimov D.D. — collection and processing of material;
Smolyankin D.O. — collection and processing of material;
Valova Y.V. — collection and processing of material, statistical processing;
Karimov D.O. — concept and design of the study, statistical processing;
Muhammadieva G.F. — editing;
Repina E.F. — concept and design of the study;
Akhmadeev A.R. — collection and processing of material.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.

Conflict of interest. The authors declare no conflict of interest.

Acknowledgement. Industry research program of Rospotrebnadzor for 2021-2025 “Scientific substantiation of the national system for ensuring sanitary and epidemiological well-being, managing health risks and improving the quality of life of the population of Russia”, on the topic: “Studying the impact of chemical production factors in conditions of chronic stress” No. NIOKTR I124021200153 -3.

Received: February 20, 2024 / Accepted: March 11 2024 / Published: April 10, 2024

 

About the authors

Alina A. Gizatullina

Ufa Research Institute of Occupational Health and Human Ecology

Author for correspondence.
Email: alinagisa@yandex.ru
ORCID iD: 0000-0002-7321-0864

Jr. researcher of the Dept. of toxicology and genetics with the experimental laboratory animal clinic of the Ufa Research Institute of Occupational Medicine and Human Ecology, Ufa, 450106, Russian Federation

e-mail: alinagisa@yandex.ru

Russian Federation

Nadezhda Yu. Khusnutdinova

Ufa Research Institute of Occupational Health and Human Ecology

Email: h-n-yu@yandex.ru
ORCID iD: 0000-0001-5596-8180

Researcher of the Dept. of toxicology and genetics with the experimental laboratory animal clinic of the Ufa Research Institute of Occupational Medicine and Human Ecology, Ufa, 450106, Russian Federation

e-mail: h-n-yu@yandex.ru

Russian Federation

Denis D. Karimov

Ufa Research Institute of Occupational Health and Human Ecology

Email: lich-tsar@mail.ru
ORCID iD: 0000-0002-1962-2323

MD, PhD, Senior researcher of the Dept. of toxicology and genetics with an experimental laboratory animal clinic of the Ufa Research Institute of Occupational Medicine and Human Ecology, Ufa, 450106, Russian Federation

e-mail: lich-tsar@mail.ru

Russian Federation

Denis A. Smolyankin

Ufa Research Institute of Occupational Health and Human Ecology

Email: smolyankin.denis@yandex.ru
ORCID iD: 0000-0002-7957-2399

Jr. researcher of the Dept. of toxicology and genetics with an experimental laboratory animal clinic of the Ufa Research Institute of Occupational Medicine and Human Ecology, Ufa, 450106, Russian Federation

e-mail: smolyankin.denis@yandex.ru

Russian Federation

Yana V. Valova

Ufa Research Institute of Occupational Health and Human Ecology

Email: Q.juk@yandex.ru
ORCID iD: 0000-0001-6605-9994

Jr. researcher of the Dept. of toxicology and genetics with an experimental laboratory animal clinic of the Ufa Research Institute of Occupational Medicine and Human Ecology, Ufa, 450106, Russian Federation

e-mail: Q.juk@yandex.ru

Russian Federation

Denis O. Karimov

Ufa Research Institute of Occupational Health and Human Ecology

Email: karimovdo@gmail.com
ORCID iD: 0000-0003-0039-6757

MD, PhD, head of the Dept. of toxicology and genetics with an experimental laboratory animal clinic of the Ufa Research Institute of Occupational Medicine and Human Ecology, Ufa, 450106, Russian Federation

e-mail: karimovdo@gmail.com

Russian Federation

Guzel F. Muhammadieva

Ufa Research Institute of Occupational Health and Human Ecology

Email: ufniimt@mail.ru
ORCID iD: 0000-0002-7456-4787

MD, PhD, Senior researcher of the Dept. of toxicology and genetics with an experimental laboratory animal clinic of the Ufa Research Institute of Occupational Medicine and Human Ecology, Ufa, 450106, Russian Federation

e-mail: ufniimt@mail.ru

Russian Federation

Elvira F. Repina

Ufa Research Institute of Occupational Health and Human Ecology

Email: e.f.repina@bk.ru
ORCID iD: 0000-0001-8798-0846

MD, PhD, senior researcher of the Dept. of toxicology and genetics with an experimental laboratory animal clinic of the Ufa Research Institute of Occupational Medicine and Human Ecology, Ufa, 450106, Russian Federation

e-mail: e.f.repina@bk.ru

Russian Federation

Aidar R. Akhmadeev

Ufa Research Institute of Occupational Health and Human Ecology

Email: dgaar87@gmail.com
ORCID iD: 0000-0001-7309-4990

Jr. researcher of the Dept. of toxicology and genetics with an experimental laboratory animal clinic of the Ufa Research Institute of Occupational Medicine and Human Ecology, Ufa, 450106, Russian Federation

e-mail: dgaar87@gmail.com

Russian Federation

References

  1. Hellhammer D.H., Hellhammer J., eds. Stress: the Brain-Body Connection. Karger Medical and Scientific Publishers; 2008.
  2. McEwen B.S. The brain is the central organ of stress and adaptation. Neuroimage. 2009; 47(3): 911–3. https://doi.org/10.1016/j.neuroimage.2009.05.071
  3. Sibgatullin I.Ya., Fatkhutdinova L.M. Methods of prevention of occupational stress and emotional burnout of medical workers (literature review). Meditsina truda i ekologiya cheloveka. 2022; (4): 20–33. (in Russian)
  4. Akarachkova E.S., Baydauletova A.I., Belyaev A.A., Blinov D.V., Gromova O.A., Dulaeva M.S., et al. Stress: Causes and Consequences, Treatment and Prevention. Clinical Recommendations [Stress: prichiny i posledstviya, lechenie i profilaktika. Klinicheskie rekomendatsii]. St. Petersburg: Skifiya-print; 2020. (in Russian)
  5. Kershaw K.N., Lane-Cordova A.D., Carnethon M.R., Tindle H.A., Liu K. Chronic stress and endothelial dysfunction: The Multi-Ethnic Study of Atherosclerosis (MESA). Am. J. Hypertens. 2017; 30(1): 75–80. https://doi.org/10.1093/ajh/hpw103
  6. Yao B.C., Meng L.B., Hao M.L., Zhang Y.M., Gong T., Guo Z.G. Chronic stress: a critical risk factor for atherosclerosis. J. Int. Med. Res. 2019; 47(4): 1429–40. https://doi.org/10.1177/0300060519826820
  7. Dai S., Mo Y., Wang Y., Xiang B., Liao Q., Zhou M., et al. Chronic stress promotes cancer development. Front. Oncol. 2020; 10: 1492. https://doi.org/10.3389/fonc.2020.01492
  8. Woo E., Sansing L.H., Arnsten A.F.T., Datta D. Chronic stress weakens connectivity in the prefrontal cortex: architectural and molecular changes. Chronic Stress (Thousand Oaks). 2021; 5: 24705470211029254. https://doi.org/10.1177/24705470211029254
  9. Tarskikh M.M., Klimatskaya L.G., Kolesnikov S.I. Pathogenesis of neurotoxicity of acrylates acrylonitrile and acrylamide: from cell to organism. Bull. Exp. Biol. Med. 2013; 155(4): 451–3. https://doi.org/10.1007/s10517-013-2175-4
  10. Uthra C., Shrivastava S., Jaswal A., Sinha N., Reshi M.S., Shukla S. Therapeutic potential of quercetin against acrylamide induced toxicity in rats. Biomed. Pharmacother. 2017; 86: 705–14. https://doi.org/10.1016/j.biopha.2016.12.065
  11. Gökmen V. Preface. In: Acrylamide in Food. Analysis, Content and Potential Health Effects. Academic Press; 2016: 19–20. https://doi.org/10.1016/B978-0-12-802832-2.05001-4
  12. Bušová M., Bencko V., Veszelits Laktičová K., Holcátová I., Vargová M. Risk of exposure to acrylamide. Cent. Eur. J. Public Health. 2020; 28(Suppl.): S43–6. https://doi.org/10.21101/cejph.a6177
  13. Bin-Jumah M., Abdel-Fattah A.M., Saied E.M., El-Seedi H.R., Abdel-Daim M.M. Acrylamide-induced peripheral neuropathy: manifestations, mechanisms, and potential treatment modalities. Environ. Sci. Pollut. Res. Int. 2021; 28(11): 13031–46. https://doi.org/10.1007/s11356-020-12287-6
  14. Arenas M.C., Daza-Losada M., Vidal-Infer A., Aguilar M.A., Miñarro J., Rodríguez-Arias M. Capacity of novelty-induced locomotor activity and the hole-board test to predict sensitivity to the conditioned rewarding effects of cocaine. Physiol. Behav. 2014; 133: 152–60. https://doi.org/10.1016/j.physbeh.2014.05.028
  15. Pisula W., Modlinska K., Goncikowska K., Chrzanowska A. Can the hole-board test predict a rat’s exploratory behavior in a free-exploration test? Animals (Basel). 2021; 11(4): 1068. https://doi.org/10.3390/ani11041068
  16. Gabay I.A., Mukhachev E.V., Mikhaylova K.A., Nosov V.N. Approbation of a method for assessing the horizontal motor activity of white laboratory rats using the automated «Open Field» installation. Obshchestvo. Sreda. Razvitie. 2011; (3): 223–6. https://elibrary.ru/oijfhl (in Russian)
  17. Kade A.Kh., Kravchenko S.V., Trofimenko A.I., Polyakov P.P., Lipatova A.S., Anan’eva E.I., et al. Modern methods of anxiety assessment of rodents by tests based on unconditional behavior models. Kubanskiy nauchnyy meditsinskiy vestnik. 2018; 25(6): 171–6. https://doi.org/10.25207/1608-6228-2018-25-6-171-176 https://elibrary.ru/yrnbud (in Russian)
  18. Üremiş M.M., Üremiş N., Gül M., Gül S., Çiğremiş Y., Durhan M., et al. Acrylamide, applied during pregnancy and postpartum period in offspring rats, significantly disrupted myelination by decreasing the levels of myelin-related proteins: MBP, MAG, and MOG. Neurochem. Res. 2024; 49(3): 617–35. https://doi.org/10.1007/s11064-023-04053-0
  19. Quan W., Li M., Jiao Y., Zeng M., He Z., Shen Q., et al. Effect of dietary exposure to acrylamide on diabetes-associated cognitive dysfunction from the perspectives of oxidative damage, neuroinflammation, and metabolic disorders. J. Agric. Food Chem. 2022; 70(14): 4445–56. https://doi.org/10.1021/acs.jafc.2c00662
  20. Molina P., Andero R., Armario A. Restraint or immobilization: A comparison of methodologies for restricting free movement in rodents and their potential impact on physiology and behavior. Neurosci. Biobehav. Rev. 2023; 151: 105224. https://doi.org/10.1016/j.neubiorev.2023.105224
  21. Mosolov S.N., Fedorova E.Yu. Risk of developing cardiovascular disease in bipolar disorder. Biological factors and therapy. Terapevticheskij arhiv. 2022; 94(4): 579–83. https://doi.org/10.26442/00403660.2022.04.201455 (in Russian)
  22. Armario A., Labad J., Nadal R. Focusing attention on biological markers of acute stressor intensity: Empirical evidence and limitations. Neurosci. Biobehav. Rev. 2020; 111: 95–103. https://doi.org/10.1016/j.neubiorev.2020.01.013
  23. Himanshu, Dharmila, Sarkar D., Nutan. A review of behavioral tests to evaluate different types of anxiety and anti-anxiety effects. Clin. Psychopharmacol. Neurosci. 2020; 18(3): 341–51. https://doi.org/10.9758/cpn.2020.18.3.341
  24. Casarrubea M., Di Giovanni G., Aiello S., Crescimanno G. The hole-board apparatus in the study of anxiety. Physiol. Behav. 2023; 271: 114346. https://doi.org/10.1016/j.physbeh.2023.114346
  25. Hughes R.N. Neotic preferences in laboratory rodents: issues, assessment and substrates. Neurosci. Biobehav. Rev. 2007; 31(3): 441–64. https://doi.org/10.1016/j.neubiorev.2006.11.004
  26. Brown G.R., Nemes C. The exploratory behaviour of rats in the hole-board apparatus: is head-dipping a valid measure of neophilia? Behav. Process. 2008; 78(3): 442–8. https://doi.org/10.1016/j.beproc.2008.02.019
  27. Pan M.M., Wang Q.Y., Hou J.L., Zhang T., Jiang Y., Yang L.P. Effects of umbilical moxibustion on phobic behavior and monoamine neurotransmitters in stress-model rats. Zhongguo Zhen Jiu. 2023; 43(2): 191–6. https://doi.org/10.13703/j.0255-2930.20211123-k0004 (in Chinese)
  28. Bukia N., Butskhrikidze M., Machavariani L., Svanidze M., Nozadze T. Gender related differences in sex hormone-mediated anxiolytic effects of electromagnetic stimulation during immobilization stress. Georgian Med. News. 2022; (323): 131–7.
  29. Hou J., Chen Y., Ma D., Wang C., Jin H., An Y., et al. Effect of chronic emotional stress induced by empty bottle stimulation on inflammatory factors in rats with acute myocardial infarction: analysis of the CXCL12/CXCR4 axis. Nan Fang Yi Ke Da Xue Xue Bao. 2020; 40(5): 624–31. https://doi.org/10.12122/j.issn.1673-4254.2020.05.03 (in Chinese)
  30. Borisova-Nenova V., Eftimov M., Valcheva-Kuzmanova S. Behavioral effects of Chaenomeles maulei fruit juice in rats with impaired circadian rhythm. Folia Med. (Plovdiv). 2023; 65(1): 155–60. https://doi.org/10.3897/folmed.65.e71854
  31. Wang A.L., Micov V.B., Kwarteng F., Wang R., Hausknecht K.A., Oubraim S., et al. Prenatal ethanol exposure leads to persistent anxiety-like behavior during adulthood indicated by reduced horizontal and vertical exploratory behaviors. Front. Neurosci. 2023; 17: 1163575. https://doi.org/10.3389/fnins.2023.1163575
  32. Oshiro W.M., McDaniel K.L., Beasley T.E., Moser V., Herr D.W. Impacts of a perinatal exposure to manganese coupled with maternal stress in rats: Learning, memory and attentional function in exposed offspring. Neurotoxicol. Teratol. 2022; 91: 107077. https://doi.org/10.1016/j.ntt.2022.107077
  33. McBlane J.W., Handley S.L. Effects of two stressors on behaviour in the elevated X-maze: preliminary investigation of their interaction with 8-OH-DPAT. Psychopharmacology (Berl.). 1994; 116(2): 173–82. https://doi.org/10.1007/BF02245060
  34. Bakhtiyarova Sh.K., Kapysheva U.N., Ablaykhanova N.T., Baimbetova A.K., Zhaksymov B.I., Korganbaeva A.A., et al. Behavior of animals in various tests. Mezhdunarodnyy zhurnal prikladnykh i fundamental’nykh issledovaniy. 2017; (8-1): 92–6. https://elibrary.ru/zcispb (in Russian)
  35. Manukhina E.B., Tseilikman V.E., Tseilikman O.B., Komelkova M.V., Kondashevskaya M.V., Goryacheva A.V., et al. Intermittent hypoxia improves behavioral and adrenal gland dysfunction induced by posttraumatic stress disorder in rats. J. Appl. Physiol. (1985). 2018; 125(3): 931–7. https://doi.org/10.1152/japplphysiol.01123.2017
  36. Lelevich V.V., Sheybak V.M., Ledneva I.O., Petushok N.E. Biological Chemistry: Workshop for Students Studying in the Specialty 1-79 01 02 «Pediatrics» [Biologicheskaya khimiya: praktikum dlya studentov, obuchayushchikhsya po spetsial’nosti 1-79 01 02 «Pediatriya»]. Grodno; 2022. (in Russian)
  37. Zhao H., Song L., Qiang Y., Liu H.R., Qiu F.Y., Li X.Z., et al. Association between occupational stress and aminotransferase activity in patients with metabolic syndrome. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2016; 34(12): 911–6. https://doi.org/10.3760/cma.j.issn.1001-9391.2016.12.007 (in Chinese)
  38. Martín M.G., Pfrieger F., Dotti C.G. Cholesterol in brain disease: sometimes determinant and frequently implicated. EMBO Rep. 2014; 15(10): 1036–52. https://doi.org/10.15252/embr.201439225
  39. Cheon S.Y. Impaired cholesterol metabolism, neurons, and neuropsychiatric disorders. Exp. Neurobiol. 2023; 32(2): 57–67. https://doi.org/10.5607/en23010
  40. Dayeh M.A., Livadiotis G., Aminian F., Cheng K.H., Roberts J.L., Viswasam N., et al. Effects of cholesterol in stress-related neuronal death – a statistical analysis perspective. Int. J. Mol. Sci. 2020; 21(8): 2905. https://doi.org/10.3390/ijms21082905
  41. Zarrouk A., Hammouda S., Ghzaiel I., Hammami S., Khamlaoui W., Ahmed S.H., et al. Association between oxidative stress and altered cholesterol metabolism in Alzheimer’s disease patients. Curr. Alzheimer Res. 2020; 17(9): 823–34. https://doi.org/10.2174/1567205017666201203123046

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