Monitoring of Klebsiella pneumoniae as one of the criteria of microecological characterization of a water body

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Abstract

Introduction. Representatives of the family Enterobacteriaceae are ubiquitous in the environment, causing severe, including opportunistic diseases in immunocompromised individuals. The occurrence of antibiotic-sensitive bacteria of the Klebsiella genus within the recreational zone of a water body is potent of posing an epidemiologic danger to the inhabitants of the settlement.

Materials and Methods. Water samples from the surface aquifers of the Don and Temernik rivers of Rostov-on-Don served as the material for the study. The sensitivity of isolates of Kl. pneumoniae isolates to fourteen antibacterial preparations were investigated by disk-diffusion method in accordance with the requirements of MUK 4.2.1980-04 and EUCAST v. 13.0 (2023). 13.0 (2023). Carbapenemase genes VIM, IMP, NDM, KPC, OXA-48, NDM were determined using commercial diagnostic kits AmpliSense MDR MBL-FL, AmpliSense MDR KPC/OXA-48-FL, AmpliSense MDR Ab-OXA-FL.

Results. In 2022, 247 isolates of Klebsiella pneumoniae were isolated in 14 biotopes of water bodies of Rostov-on-Don. Biotopes of epidemiological significance were identified with the presence of antibiotic resistance genes in 28 isolates isolated above and below the sewage discharge site (sampling point #1 and #5) and strains isolated from the mouth of the Temernik River (sampling point #14). Phenotype of multidrug-resistant (MDR) in point No. 1 possessed 22%, point No. 5 — 26.9%, point No. 14 — 26%; phenotype of extreme drug resistance (XDR) in point No. 1 possessed 11.1 %, point No. 5 — 15.3%, point No. 14 — 13%; phenotype of pan-drug-resistant (PDR) in point No. 1 did not possess, point No. 5 — 3.8%, point No. 14 — 4.3%.

Limitations. A limitation of the study is related to the location of water sampling from the river. Don and R. Temernik on the territory of Rostov-on-Don, covering from March to November 2022. In this article, we limited ourselves to sanitary-bacteriological and molecular genetic analysis.

Conclusion. The detection of PDR, XDR, and MDR antibiotic-resistant phenotypes of Klebsiella pneumoniae, as well as the detection of resistance genes, indicates to an increased risk of water-associated acute intestinal infections, especially in immunosuppressed individuals.

Compliance with ethical standards. The study does not require the submission of the conclusion of the biomedical ethics committee.

Contribution:
Kalyuzhin A.С. — Development of the study design, analysis of the obtained data, preparation of the manuscript;
Bayrakova A.L. — study design development, analysis of the obtained data, manuscript preparation;
Morozova M.A. — analysis of the obtained data, preparation of the manuscript, editing of the manuscript;
Latyshevskaya N.I. — editing of the manuscript;
Ruzhentsova T.A. — editing of the manuscript text.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version

Conflict of interest. The authors declare no conflict of interest.

Acknowledgement. The study had no sponsorship.

Received: November 28, 2023 / Revised: February 20, 2024 / Accepted: March 11 / Published: April 10, 2024

About the authors

Alexander S. Kalyuzhin

Federal Scientific Center of Hygiene named after F.F. Erisman of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing; Volgograd State of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing Medical University” of the Ministry of Health of Russia

Author for correspondence.
Email: o.t.t.o.94@mail.ru
ORCID iD: 0000-0002-7234-6890

3rd year graduate student of the Department of general hygiene and ecology of the Volgograd State Medical University of the Ministry of Health of Russia, Volgograd, 400131, Russian Federation; junior researcher of the Dept. of Water Hygiene Institute of Complex Hygiene Problems of the Federal Scientific Center of Hygiene named after F.F. Erisman, Rospotrebnadzor, Mytishchi, 141014, Russian Federation

e-mail: o.t.t.o.94@mail.ru

Russian Federation

Alexandra L. Bayrakova

Moscow Research Institute of Epidemiology and Microbiology named after G.N. Gabrichevsky of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing

Email: alexandrabl@mail.ru

MD, PhD, senior researcher of the clinical microbiology and biotechnology laboratory of the Moscow Research Institute of Epidemiology and Microbiology named after G.N. Gabrichevsky, Rospotrebnadzor, Moscow, 125212, Russian Federation; assistant at the Russian University of Medicine

e-mail: alexandrabl@mail.ru

Russian Federation

Marina A. Morozova

Rostov Research Institute of Microbiology and Parasitology of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing

Email: morozova.q@mail.ru
ORCID iD: 0000-0003-2017-9717

MD, PhD, senior researcher of the Laboratory of Sanitary Microbiology of Water Bodies and Human Microbial Ecology of the Rostov Research Institute of Microbiology and Parasitology, Rospotrebnadzor; Rostov-on-Don, 344003, Russian Federation

e-mail: morozova.q@mail.ru

Russian Federation

Natalya I. Latyshevskaya

Volgograd State of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing Medical University” of the Ministry of Health of Russia

Email: latyshnata@mail.ru
ORCID iD: 0000-0002-8367-745X

MD, PhD, DSci., Professor, Head of the Department of general hygiene and ecology of the Volgograd State Medical University of the Ministry of Health of Russia, Volgograd, 400131, Russian Federation

e-mail: latyshnata@mail.ru

Russian Federation

Tatyana A. Ruzhentsova

Moscow Research Institute of Epidemiology and Microbiology named after G.N. Gabrichevsky of the Federal Service for Supervision in Protection of the Rights of Consumer and Man Wellbeing

Email: ruzhencova@gmail.com
ORCID iD: 0000-0002-6945-2019

MD, PhD, DSci., Professor, Deputy Director for the Clinical Work for Moscow Research Institute of Epidemiology and Microbiology named after G.N. Gabrichevsky, Rospotrebnadzor, Moscow, 125212, Russian Federation

e-mail: ruzhencova@gmail.com

Russian Federation

References

  1. Naidoo S., Olaniran A.O. Treated wastewater effluent as a source of microbial pollution of surface water resources. Int. J. Environ. Res. Public Health. 2013; 11(1): 249–70. https://doi.org/10.3390/ijerph110100249
  2. Ostroumov S.A. A biological mechanism of self-purification in natural water bodies and streams: theory and applications. Uspekhi sovremennoy biologii. 2004; 124(5): 429–42. https://elibrary.ru/oxnkwr (in Russian)
  3. Erostate M., Huneau F., Garel E., Ghiotti S., Vystavna Y., Garrido M., et al. Groundwater dependent ecosystems in coastal Mediterranean regions: Characterization, challenges and management for their protection. Water Res. 2020; 172: 115461. https://doi.org/10.1016/j.watres.2019.115461
  4. Sboychakov V.B., Kletsko L.I. The role of water in the transmission of infectious pathogens. In: IV Luga Scientific Readings. Modern scientific knowledge: theory and practice. Materials of the international scientific and practical conference [IV Luzhskie nauchnye chteniya. Sovremennoe nauchnoe znanie: teoriya i praktika. Materialy mezhdunarodnoy nauchno-prakticheskoy konferentsii]. St. Petersburg; 2016: 169–72. https://elibrary.ru/yfqhcp (in Russian)
  5. Edgorova N.T., Berdimurodov B.P., Ergasheva Z.N.K. The basic properties of enterbacteries meet in open water systems. Biologiya i integrativnaya meditsina. 2018; (9): 17–34. https://elibrary.ru/upktjk (in Russian)
  6. Kolychev N.M., Petrova M.I., Egorova A.S. The sanitary-microbiological analysis of potable water from the planting network of the decentralized water sources of cattle-breeding farms of the Omsk region. Vestnik Omskogo gosudarstvennogo agrarnogo universiteta. 2011; 3(3): 72–5. https://elibrary.ru/synpop (in Russian)
  7. Zagaynova A.V., Artemova T.Z., Trukhina G.M., Sukhina M.A. Hygienic standardization of water quality and safety in centralized drinking water supply systems, taking into account the modern taxonomy of microorganisms. In: Sychik S.I., ed. Health and the Environment. Collection of Materials of the International Scientific and Practical Conference [Zdorov’e i okruzhayushchaya sreda. Sbornik materialov mezhdunarodnoy nauchno-prakticheskoy konferentsii]. Minsk; 2021: 55–6. https://elibrary.ru/aedrwb (in Russian)
  8. Bukharova E.V., Popkova S.M., Rakova E.B., Dzhioev Yu.P., Shabanova N.M., Ivanova E.I., et al. Microecological characteristics of Klebsiella spp. regional populations in the intestinal microbiota of Irkutsk children. Byulleten’ Vostochno-Sibirskogo nauchnogo tsentra Sibirskogo otdeleniya Rossiyskoy akademii meditsinskikh nauk. 2013; (2–2): 93–6. https://elibrary.ru/riimjz (in Russian)
  9. Zagaynova A.V., Yudin S.M., Abramov I.A., Nedachin A.E., Aslanova M.M., Lukashina M.V., et al. Determination of the list of potentially pathogenic and pathogenic microorganisms of bacterial, viral and parasitic nature circulating in waste and surface waters. Meditsinskaya parazitologiya i parazitarnye bolezni. 2021; (2): 50–63. https://doi.org/10.33092/0025-8326mp2021.2.50-63 https://elibrary.ru/bacglw (in Russian)
  10. Campbell L.M., Michaels G., Klein R.D., Roth I.L. Isolation of Klebsiella pneumoniae from lake water. Can. J. Microbiol. 1976; 22(12): 1762–7. https://doi.org/10.1139/m76-260
  11. Korobeyko E.S., Nakaznaya E.N., Ovechkina I.G. Distribution of bacteria of the Klebsiella strain in water objects and their value in developing of the water caused acute intestinal infections. In: Current Problems and Methodological Approaches to the Diagnosis, Treatment and Prevention of Animal Diseases. Materials of the International Scientific and Practical Conference [Aktual’nye problemy i metodicheskie podkhody k diagnostike, lecheniyu i profilaktike bolezney zhivotnykh. Materialy mezhdunarodnoy nauchno-prakticheskoy konferentsii]. Sett. Persianovskiy; 2019: 143–8. https://elibrary.ru/vydmyk (in Russian)
  12. Podschun R., Pietsch S., Höller C., Ullmann U. Incidence of Klebsiella species in surface waters and their expression of virulence factors. Appl. Environ. Microbiol. 2001; 67(7): 3325–7. https://doi.org/10.1128/AEM.67.7.3325-3327.2001
  13. Lartseva L.V., Obukhova O.V. Comparative assessment of the sanitary and hygienic significance of Klebsiella isolated from clinical material and hydroecosystems. Literature review. Astrakhanskiy vestnik ekologicheskogo obrazovaniya. 2020; (2): 143–54. https://doi.org/10.36698/2304-5957-2020-19-2-143-154 https://elibrary.ru/olqsut (in Russian)
  14. Lartseva L.V., Lisitskaya I.A., Obukhova O.V. Microbiocenosis of Water and Sturgeon Natural Populations of the Volga-Caspian Basin [Mikrobiotsenoz vody i osetrovykh estestvennykh populyatsiy Volgo-Kaspiyskogo basseyna]. Astrakhan’; 2020. https://elibrary.ru/zozyrw (in Russian)
  15. Men’shikov V.V., ed. Clinical Laboratory Analytics. Volume IV. Private Analytical Technologies in the Clinical Laboratory [Klinicheskaya laboratornaya analitika. Tom IV. Chastnye analiticheskie tekhnologii v klinicheskoy laboratorii]. Moscow: Agat-Med; 2003. https://elibrary.ru/ejatpq (in Russian)
  16. Shamina O.V., Samoylova E.A., Novikova I.E., Lazareva A.V. Klebsiella pneumoniae: microbiological characteristics, antibiotic resistance, and virulence. Rossiyskiy pediatricheskiy zhurnal. 2020; 23(3): 191–7. https://doi.org/10.18821/1560-9561-2020-23-3-191-197 https://elibrary.ru/fytman (in Russian)
  17. Ageevets V.A., Ageevets I.V., Sidorenko S.V. Convergence of multiple resistance and hypervirulence in Klebsiella pneumoniae. Infektsiya i immunitet. 2022; 12(3): 450–60. https://doi.org/10.15789/2220-7619-COM-1825 https://elibrary.ru/ucpmnf (in Russian)
  18. Labinskaya A.S., Blinkova L.P., Eshchina A.S., eds. Private Medical Microbiology with the Technique of Microbiological Research [Chastnaya meditsinskaya mikrobiologiya s tekhnikoy mikrobiologicheskikh issledovaniy]. Moscow: Meditsina; 2005. https://elibrary.ru/qllyhz (in Russian)
  19. Birger M.O. Handbook of Microbiological and Virological Research Methods [Spravochnik po mikrobiologicheskim i virusologicheskim metodam issledovaniya]. Moscow: Meditsina; 1982. (in Russian)
  20. Magiorakos A.P., Srinivasan A., Carey R.B., Carmeli Y., Falagas M.E., Giske C.G., et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin. Microbiol. Infect. 2012; 18(3): 268–81. https://doi.org/10.1111/j.1469-0691.2011.03570.x
  21. Rakhmanin Yu.A., Ivanova L.V., Artemova T.Z., Gipp E.K., Zagaynova A.V., Maksimkina T.N., et al. Comparative assessment of the sanitary and epidemic importance of coliform indicators of the drinking water quality. Gigiena i Sanitaria (Hygiene and Sanitation, Russian journal). 2019; 98(3): 237–49. https://doi.org/10.18821/0016-9900-2019-98-3-237-249 https://elibrary.ru/wxswgl (in Russian)
  22. Drake L.A., Doblin M.A., Dobb F.C. Potential microbial bioinvasions via ships’ ballast water, sediment, and biofilm. Mar. Pollut. Bull. 2007; 55(7–9): 333–41. https://doi.org/10.1016/j.marpolbul.2006.11.007
  23. Radović J.R., Rial D., Lyons B.P., Harman C., Viñas L., Beiras R., et al. Post-incident monitoring to evaluate environmental damage from shipping incidents: chemical and biological assessments. J. Environ. Manage. 2012; 109: 136–53. https://doi.org/10.1016/j.jenvman.2012.04.042
  24. Rhodes A.L., Newton R.M., Pufall A. Influences of land use on water quality of a diverse New England watershed. Environ. Sci. Technol. 2001; 35(18): 3640–5. https://doi.org/10.1021/es002052u
  25. Tapal’skiy D.V., Kozlova A.I. Sensitivity of Klebsiella pneumoniae clinical isolates with various levels of antibiotic resistance to bacteriorage preparations. Problemy zdorov’ya i ekologii. 2018; (1): 56–62. https://elibrary.ru/yuzgbf (in Russian)
  26. Lillini R., Tittarelli A., Bertoldi M., Ritchie D., Katalinic A., Pritzkuleit R., et al. Water and soil pollution: ecological environmental study methodologies useful for public health projects. A literature review. Rev. Environ. Contam. Toxicol. 2021; 256: 179–214. https://doi.org/10.1007/398_2020_58
  27. Jian Z., Zeng L., Xu T., Sun S., Yan S., Yang L., et al. Antibiotic resistance genes in bacteria: Occurrence, spread, and control. J. Basic Microbiol. 2021; 61(12): 1049–70. https://doi.org/10.1002/jobm.202100201
  28. Li L., Wu J., Lu J., Li K., Zhang X., Min X., et al. Water quality evaluation and ecological-health risk assessment on trace elements in surface water of the northeastern Qinghai-Tibet Plateau. Ecotoxicol. Environ. Saf. 2022; 241: 113775. https://doi.org/10.1016/j.ecoenv.2022.113775
  29. Volodina V.V., D’yakova S.A. Opportunistic microflora of the Caspian seal (Phoca caspica) and its habitat under anthropogenic pressure. Trudy VNIRO. 2016; 162: 87–96. https://elibrary.ru/xagklt (in Russian)
  30. Voronov Yu.V., Yakovlev S.V. Water Disposal and Wastewater Treatment [Vodootvedenie i ochistka stochnykh vod]. Moscow; 2006. (in Russian)

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